{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:47:26Z","timestamp":1777463246553,"version":"3.51.4"},"reference-count":21,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:p> Islet amyloid polypeptide (IAPP) has been shown to form amyloid deposits in pancreatic islets, thereby furthering type 2 diabetes disease progression. Further discovery of new molecules is needed to create a diverse set of molecules that impede pancreatic amyloidosis. We have recently designed and synthesized N-phenyl-N\u2032-(2-ethyl)ureas (EU) that are non-cytotoxic small molecules, to evaluate the role of the aryl-substituted moiety on the inhibition of hIAPP fibrillization. Several EUs were tested in vitro for their anti-amyloidogenic activity using the fluorometric ThT assay, the photo-induced cross-linking (PIUCP) assay, and cell survival assay in pancreatic MIN-6 cells. EU-362 and EU-418 were able to significantly inhibit the formation of hIAPP fibrils and protected cells from amyloid cytotoxic effects. Our results suggest that increasing the nucleophilic potency of the aryl moiety significantly enhances the anti-amyloidogenic activity of the molecules. <\/jats:p>","DOI":"10.1139\/cjpp-2015-0204","type":"journal-article","created":{"date-parts":[[2015,9,11]],"date-time":"2015-09-11T21:36:01Z","timestamp":1442007361000},"page":"341-346","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Discovery of ethyl urea derivatives as inhibitors of islet amyloid polypeptide fibrillization and cytotoxicity"],"prefix":"10.1139","volume":"94","author":[{"given":"Jessica S.","family":"Fortin","sequence":"first","affiliation":[{"name":"D\u00e9partement de Pathologie et de Microbiologie, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, Saint-Hyacinthe, QC J2S 2M2, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Odile","family":"Benoit-Biancamano","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pathologie et de Microbiologie, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, Saint-Hyacinthe, QC J2S 2M2, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u00e9","family":"C.-Gaudreault","sequence":"additional","affiliation":[{"name":"CR CHU de Qu\u00e9bec, Axe oncologie, H\u00f4pital Saint-Fran\u00e7ois d\u2019Assise, 10 rue de l\u2019Espinay, Qu\u00e9bec, QC G1L 3L5, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2013.01.017"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1038\/srep04267"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1021\/bi2015162"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1305517110"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.02.042"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-2549"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.3109\/13506129.2010.530008"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","unstructured":"Fortin, J.S., and Benoit-Biancamano, M.O. 2015. In Vitro Evaluation of Hypoglycemic Agents to Target Human Islet Amyloid Polypeptide: A Key Protein Involved in Amyloid Deposition and Beta-Cell Loss. Can. J. Diabetes. In Press. 10.1016\/j.jcjd.2015.01.291. 25862064.","DOI":"10.1016\/j.jcjd.2015.01.291"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2007.04.028"},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2008.06.006"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.12.062"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.02.010"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.3109\/13506129.2014.937857"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1021\/bi702518m"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s10637-009-9222-z"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2011.10.023"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1039\/c3cp44412k"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.3109\/13506129.2014.964858"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00042.2009"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.03.010"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.11.050"}],"container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/cjpp-2015-0204","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/cjpp-2015-0204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T16:14:26Z","timestamp":1751472866000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/cjpp-2015-0204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1139\/cjpp-2015-0204"],"URL":"https:\/\/doi.org\/10.1139\/cjpp-2015-0204","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3]]},"assertion":[{"value":"2015-05-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}