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The present study was undertaken to explore the underlying mechanisms of exogenous H<jats:sub>2<\/jats:sub>S on autophagy regulation in cerebral ischemia. The effects and its connection with autophagy of NaHS, a H<jats:sub>2<\/jats:sub>S donor, were observed through neurological deficits and cerebral infarct volume in middle cerebral artery occlusion (MCAO) mice; autophagy-related proteins and autophagy complex levels in the ischemic hemisphere were detected with Western blot assay. Compared with the model group, NaHS significantly decreased infarct volume and improved neurological deficits; rapamycin, an autophagy activator, abolished the effect of NaHS; NaHS decreased the expression of LC3-II and up-regulated p62 expression in the ischemic cortex 24 h after ischemia. However, NaHS did not significantly influence Beclin-1 expression. H<jats:sub>2<\/jats:sub>S has a neuroprotective effect on ischemic injury in MCAO mice; this effect is associated with its influence in down-regulating autophagosome accumulation. <\/jats:p>","DOI":"10.1139\/cjpp-2016-0100","type":"journal-article","created":{"date-parts":[[2016,6,23]],"date-time":"2016-06-23T00:53:11Z","timestamp":1466643191000},"page":"1187-1192","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":23,"title":["Exogenous hydrogen sulfide attenuates cerebral ischemia\u2013reperfusion injury by inhibiting autophagy in mice"],"prefix":"10.1139","volume":"94","author":[{"given":"Mengyang","family":"Shui","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China."},{"name":"Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China."},{"name":"Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanjun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China."},{"name":"Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinye","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China."},{"name":"Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-016-1968-8"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046092"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199607000-00010"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-328-8_13"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23427"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2015.07.005"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","unstructured":"Kamat, P.K., Kalani, A., and Tyagi, N. 2015. 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