{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T03:39:49Z","timestamp":1762659589613,"version":"3.41.0"},"reference-count":25,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:p> Cardiac hypertrophy often refers to the abnormal growth of heart muscle through a variety of factors. The mechanisms of cardiomyocyte hypertrophy have been extensively investigated using neonatal rat cardiomyocytes treated with phenylephrine. \u03b1-Enolase is a glycolytic enzyme with \u201cmultifunctional jobs\u201d beyond its catalytic activity. Its possible contribution to cardiac dysfunction remains to be determined. The present study aimed to investigate the change of \u03b1-enolase during cardiac hypertrophy and explore its role in this pathological process. We revealed that mRNA and protein levels of \u03b1-enolase were significantly upregulated in hypertrophic rat heart induced by abdominal aortic constriction and in phenylephrine-treated neonatal rat cardiomyocytes. Furthermore, knockdown of \u03b1-enolase by RNA interference in cardiomyocytes mimicked the hypertrophic responses and aggravated phenylephrine-induced hypertrophy without reducing the total glycolytic activity of enolase. In addition, knockdown of \u03b1-enolase led to an increase of GATA4 expression in the normal and phenylephrine-treated cardiomyocytes. Our results suggest that the elevation of \u03b1-enolase during cardiac hypertrophy is compensatory. It exerts a catalytic independent role in protecting cardiomyocytes against pathological hypertrophy. <\/jats:p>","DOI":"10.1139\/cjpp-2017-0282","type":"journal-article","created":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T21:36:36Z","timestamp":1505424996000},"page":"352-358","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Upregulation of \u03b1-enolase protects cardiomyocytes from phenylephrine-induced hypertrophy"],"prefix":"10.1139","volume":"96","author":[{"given":"Si","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Higher Education Mega Center, 132 East Wai-huan Road, Guangzhou, 510006 Guangdong, P.R. China."},{"name":"Department of Pharmacy, School of Medicine, Guangxi University of Science and Technology, 257 Liu-shi Road, Liuzhou, 545005 Guangxi, P.R. 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