{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T17:11:13Z","timestamp":1774458673010,"version":"3.50.1"},"reference-count":34,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:p> The purpose of this study was to determine the relationship of K<jats:sub>Ca<\/jats:sub> channels to endothelium-dependent hyperpolarizing factor (EDHF)-mediated relaxation induced by acetylcholine (ACh) in the superior mesenteric arteries of 7-month-old spontaneously hypertensive rats (SHR). Upon inhibition of nitric oxide synthase and cyclooxygenase, ACh-induced EDHF-mediated relaxation was found to be weaker in SHR than in age-matched Wistar Kyoto rats (WKY). These relaxations in both group were attenuated by combined treatment with small-conductance and intermediate-conductance Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup> channels (SK<jats:sub>Ca<\/jats:sub> and IK<jats:sub>Ca<\/jats:sub>) inhibitors, with the exception of relaxation resistant to inhibition of these channels in SHR (vs. WKY). Treatment with large-conductance Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup> channels (BK<jats:sub>Ca<\/jats:sub>) inhibitor specifically attenuated relaxation in SHR, but not in WKY. Protein expression of IK<jats:sub>Ca<\/jats:sub> and SK<jats:sub>Ca<\/jats:sub> in the arteries did not differ between the 2 groups, whereas ratio of slo\u03b21 subunit to \u03b1 subunit of BK<jats:sub>Ca<\/jats:sub> was increased in SHR (vs. WKY). These results suggest that EDHF-mediated relaxations in superior mesenteric arteries are impaired in SHR, and utilize components of BK<jats:sub>Ca<\/jats:sub> in addition to SK<jats:sub>Ca<\/jats:sub>\/IK<jats:sub>Ca<\/jats:sub> channel activities, that the increased participation of BK<jats:sub>Ca<\/jats:sub> may be attributable to alterations in \u03b1 and slo\u03b21 subunit ratio, and that components unrelated to K<jats:sub>Ca<\/jats:sub> activity may also contribute to the difference between SHR and WKY arteries. <\/jats:p>","DOI":"10.1139\/cjpp-2017-0557","type":"journal-article","created":{"date-parts":[[2018,3,20]],"date-time":"2018-03-20T21:38:07Z","timestamp":1521581887000},"page":"839-844","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Differential participation of calcium-activated potassium channel in endothelium-dependent hyperpolarization-type relaxation in superior mesenteric arteries of spontaneously hypertensive rats"],"prefix":"10.1139","volume":"96","author":[{"given":"Makoto","family":"Ando","sequence":"first","affiliation":[{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."},{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."},{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shota","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."},{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maika","family":"Iguchi","sequence":"additional","affiliation":[{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."},{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumiko","family":"Taguchi","sequence":"additional","affiliation":[{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."},{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuneo","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."},{"name":"Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1038\/sj.bjp.0707222","volume":"151","author":"Absi M.","year":"2007","journal-title":"Br. 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