{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T06:49:36Z","timestamp":1781246976957,"version":"3.54.1"},"reference-count":43,"publisher":"Canadian Science Publishing","issue":"12","license":[{"start":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T00:00:00Z","timestamp":1543622400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:p> The present study was designed to investigate the effects of calycosin on hepatic stellate cell (HSC) function and to explore whether the drug exerts its effect through the estrogen receptor. HSC proliferation and migration were measured by MTT assay and transwell chamber assay, respectively. The mRNA and protein expression of \u03b1-SMA, COL-I, and ER\u03b2 were detected by real-time PCR and Western blotting. The co-localization and expression of \u03b1-SMA and ER\u03b2 protein were detected by immunofluorescence. All the studies were investigated in the absence or presence of ICI 182,780. The results showed that calycosin inhibited the proliferation of activated HSCs and remarkably inhibited HSC migration. Calycosin significantly reduced the expression of \u03b1-SMA and COL-I in activated HSCs. However, with co-treatment with ICI 182,780, the inhibitory effect of calycosin against the above effects was strongly negated. Importantly, calycosin significantly downregulated the expression of ER\u03b2 protein, while co-treatment with ICI 182,780 partially reversed the ER\u03b2 downregulation. In addition, \u03b1-SMA decreased with the decrease of ER\u03b2 expression and the subtype of ER\u03b2 on HSC is ER\u03b2<jats:sub>5<\/jats:sub>. In conclusion, calycosin inhibits proliferation, activation, and migration of TGF-\u03b2<jats:sub>1<\/jats:sub>-induced HSCs. The effect may be related to binding and downregulation of ER\u03b2<jats:sub>5<\/jats:sub>. <\/jats:p>","DOI":"10.1139\/cjpp-2018-0474","type":"journal-article","created":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T21:37:00Z","timestamp":1539380220000},"page":"1268-1275","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":24,"title":["Inhibition effect of phytoestrogen calycosin on TGF-\u03b2<sub>1<\/sub>-induced hepatic stellate cell activation, proliferation, and migration via estrogen receptor \u03b2"],"prefix":"10.1139","volume":"96","author":[{"given":"Tan","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."},{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."},{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."},{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."},{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guannan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."},{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."},{"name":"Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, 230032, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22721"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24282"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-014-1483-3"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2006.101931"},{"issue":"4","key":"refg5\/ref5","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1097\/MEG.0000000000000567","volume":"28","author":"Corsi D.J.","year":"2016","journal-title":"Eur. 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