{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T01:07:43Z","timestamp":1782436063940,"version":"3.54.5"},"reference-count":46,"publisher":"Canadian Science Publishing","issue":"7","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:p> We tested the hypothesis that ethanol consumption would aggravate the renal damage induced by cyclophosphamide (CYP). Male C57BL\/6 J mice from control (n\u00a0= 8) and CYP (n\u00a0= 12) groups had free access to filtered water and standard rodent chow for 12\u00a0weeks. Then, 24\u00a0h before euthanasia mice received an intraperitoneal injection of saline or CYP (300\u00a0mg\/kg). Mice from ethanol (n\u00a0= 8) and CYP\u00a0+ ethanol (n\u00a0= 12) groups had free access to increasing doses of ethanol for 12\u00a0weeks. Twenty-four hours before euthanasia, mice from ethanol and CYP\u00a0+ ethanol groups received an intraperitoneal injection of saline or CYP, respectively. Ethanol, CYP, or the association of both drugs augmented serum levels of creatinine and increased the levels of superoxide ([Formula: see text]) generation and thiobarbituric acid reactive substances in the renal cortex. Upregulation of Nox4 and increased activity of superoxide dismutase were detected in the renal cortex of mice treated with ethanol, CYP, or the combination of these drugs; however, these molecular alterations induced by CYP were not potentiated by ethanol consumption. Our findings revealed that chronic ethanol consumption had no potentiating effect on the nephrotoxic effects displayed by CYP. It is possible that the combination of these drugs showed no synergistic effect because they share the same molecular mechanisms of renal toxicity. <\/jats:p>","DOI":"10.1139\/cjpp-2020-0246","type":"journal-article","created":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T16:38:34Z","timestamp":1605112714000},"page":"744-751","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Ethanol and cyclophosphamide induce similar nephrotoxic effects: possible role for Nox4 and superoxide"],"prefix":"10.1139","volume":"99","author":[{"given":"Arthur H.","family":"Sousa","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Farmacologia, DEPCH, Escola de Enfermagem de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (USP), Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel T.","family":"do 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