{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T04:19:18Z","timestamp":1751516358246,"version":"3.41.0"},"reference-count":31,"publisher":"Canadian Science Publishing","issue":"2","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:p> Activation of the parasympathetic nervous system has been reported to have an antiarrhythmic role during ischemia\u2013reperfusion injury by decreasing the arrhythmia triggers. Furthermore, it was reported that the parasympathetic neurotransmitter acetylcholine is able to modulate the ATP-dependent potassium current (I <jats:sub>K-ATP<\/jats:sub>), a crucial current activated during hypoxia. However, the possible significance of this current modulation in the antiarrhythmic mechanism is not fully clarified. Action potentials were measured using the conventional microelectrode technique from canine left ventricular papillary muscle and free-running Purkinje fibers, under normal and hypoxic conditions. Ionic currents were measured using the whole-cell configuration of the patch-clamp method. Acetylcholine at 5\u00a0\u03bcmol\/L did not influence the action potential duration (APD) either in Purkinje fibers or in papillary muscle preparations. In contrast, it significantly lengthened the APD and suppressed the Purkinje\u2013ventricle APD dispersion when it was administered after 5\u00a0\u03bcmol\/L pinacidil application. Carbachol at 3\u00a0\u03bcmol\/L reduced the pinacidil-activated I <jats:sub>K-ATP<\/jats:sub> under voltage-clamp conditions. Acetylcholine lengthened the ventricular action potential under simulated ischemia condition. In this study, we found that acetylcholine inhibits the I <jats:sub>K-ATP<\/jats:sub> and thus suppresses the ventricle\u2013Purkinje APD dispersion. We conclude that parasympathetic tone may reduce the arrhythmogenic substrate exerting a complex antiarrhythmic mechanism during hypoxic conditions. <\/jats:p>","DOI":"10.1139\/cjpp-2020-0408","type":"journal-article","created":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T21:37:19Z","timestamp":1606426639000},"page":"247-253","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Muscarinic agonists inhibit the ATP-dependent potassium current and suppress the ventricle\u2013Purkinje action potential dispersion"],"prefix":"10.1139","volume":"99","author":[{"given":"Tibor","family":"Magyar","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tam\u00e1s","family":"\u00c1rp\u00e1dffy-Lovas","sequence":"additional","affiliation":[{"name":"Department of 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