{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T04:14:36Z","timestamp":1751516076493,"version":"3.41.0"},"reference-count":29,"publisher":"Canadian Science Publishing","issue":"10","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:p> Metabolic side effects of atypical antipsychotics are an important cause of deterioration of cognitive function and failure of drug adherence. The antifatty effect trypsin\/chymotrypsin (T\/C) and their mechanisms of action remain unclear. To investigate possible therapeutic effect of T\/C in rat model of chronic olanzapine (OLZ) \u2013 induced hepatic steatosis. Twenty rats were divided into two groups: control (C), given distilled water, and O, given 1\u00a0mg\/kg of OLZ orally daily for 7 weeks. Then, both groups were given T\/C 3 enzyme activity unit (EAU)\/kg orally as an add-on treatment daily for the next 5 weeks and were named T\/C or T\/C+O groups. Rat performance in radial arm water maze was tested twice before and after T\/C treatment. We measured liver enzymes, alpha-1 antitrypsin, albumin, total protein, direct and total bilirubin, inflammatory cytokines, and lipoprotein serum levels. Liver samples were collected for histopathology and Ki67 expression. The T\/C add-on caused significant reduction in OLZ-induced elevation of alanine transaminase (ALT; P\u00a0&lt; 0.01), aspartate transaminase (AST; P\u00a0&lt; 0.001), alkaline phosphatase (ALP; P\u00a0&lt; 0.05), total cholesterol (Tc; P\u00a0&lt; 0.01), low-density lipoproteins (LDL-c; P\u00a0&lt; 0.05), steatosis score (P\u00a0&lt; 0.001), hepatocyte necrosis (P\u00a0&lt; 0.01), and significantly increased Ki67 expression (P\u00a0&lt; 0.01). The T\/C add-on to OLZ provided protection against hepatic steatosis, elevated enzymes, and disturbed lipid profile and increased Ki67 without disturbing memory function. <\/jats:p>","DOI":"10.1139\/cjpp-2021-0075","type":"journal-article","created":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T17:41:26Z","timestamp":1630604486000},"page":"1088-1096","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Hepatoprotective effect of trypsin\/chymotrypsin against olanzapine-induced non-alcoholic steatohepatitis in rats"],"prefix":"10.1139","volume":"99","author":[{"given":"Ghada S.","family":"Mahmoud","sequence":"first","affiliation":[{"name":"Departments of Medical Physiology, Faculty of Medicine, Assiut University, Assiut, Egypt."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghada","family":"Hosny","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sally A.","family":"Sayed","sequence":"additional","affiliation":[{"name":"Departments of Medical Physiology, Faculty of Medicine, Assiut University, Assiut, Egypt."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.275"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1111\/liv.13643"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1002\/1099-081X(199911)20:8<369::AID-BDD200>3.0.CO;2-6"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1002\/hep4.1381"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2014-0214-RA"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2010.6"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110912"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1038\/sj.npp.1300757"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.6.2248.bloodjournal8662248"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.3390\/children2030358"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1016\/S0305-4179(97)00080-6"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20092369"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.ndt.a027510"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-36930-y"},{"issue":"3","key":"refg15\/ref15","first-page":"64","volume":"11","author":"Mahmoud G.S.","year":"2019","journal-title":"Int. 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