{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:15:09Z","timestamp":1783469709439,"version":"3.55.0"},"reference-count":49,"publisher":"Canadian Science Publishing","issue":"10","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:p> Overexpression of the inducible isoform of the enzyme nitric oxide synthase (iNOS) has been associated to pathological processes in the kidney. Ethanol consumption induces the renal expression of iNOS; however, the contribution of this enzyme to the deleterious effects of ethanol in the kidney remains elusive. We examined whether iNOS plays a role in the renal dysfunction and oxidative stress induced by ethanol consumption. With this purpose, male C57BL\/6 wild-type (WT) or iNOS-deficient (iNOS<jats:sup>\u2013\/\u2013<\/jats:sup>) mice were treated with ethanol (20% v\/v) for 10 weeks. Treatment with ethanol increased the expression of Nox4 as well as the concentration of thiobarbituric acid reactive substances and the levels of tumor necrosis factor \u03b1 in the renal cortex of WT but not iNOS<jats:sup>\u2013\/\u2013<\/jats:sup> mice. Augmented serum levels of creatinine and increased systolic blood pressure were found in WT and iNOS<jats:sup>\u2013\/\u2013<\/jats:sup> mice treated with ethanol. WT mice treated with ethanol showed increased production of reactive oxygen species and myeloperoxidase activity, but these responses were attenuated in iNOS<jats:sup>\u2013\/\u2013<\/jats:sup> mice. We concluded that iNOS played a role in ethanol-induced oxidative stress and pro-inflammatory cytokine production in the kidney. These are mechanisms that may contribute to the renal toxicity induced by ethanol. <\/jats:p>","DOI":"10.1139\/cjpp-2021-0108","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T21:39:09Z","timestamp":1619127549000},"page":"1016-1025","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Inducible nitric oxide synthase (iNOS) mediates ethanol-induced redox imbalance and upregulation of inflammatory cytokines in the kidney"],"prefix":"10.1139","volume":"99","author":[{"given":"Carla B.P.","family":"da Silva","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Farmacologia, DEPCH, Escola de Enfermagem de Ribeir\u00e3o Preto, USP, Ribeir\u00e3o Preto, SP, Brazil."},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Toxicologia, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo (USP), Ribeir\u00e3o Preto, SP, 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