{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T04:43:25Z","timestamp":1794026605843,"version":"4.8.0"},"reference-count":84,"publisher":"Canadian Science Publishing","issue":"11","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:p> Type-2 diabetes (T2D) is associated with liver toxicity. L-ergothioneine (L-egt) has been reported to reduce toxicity in tissues exposed to injury, while metformin is commonly prescribed to manage T2D. Hence, this study evaluates the hepatoprotective role of L-egt, with or without metformin, in T2D male rats. A total of 36 adult male Sprague\u2013Dawley rats were randomly divided into non-diabetic (n\u00a0= 12) and diabetic (n\u00a0= 24) groups. After induction of diabetes, animals were divided into six groups (n = 6) and treated orally either with deionized water, L-egt (35\u00a0mg\/kg bodyweight (bwt)), metformin (500\u00a0mg\/kg bwt), or a combination of L-egt and metformin for 7 weeks. Body weight and blood glucose were monitored during the experiment. Thereafter, animals were euthanized and liver tissue was excised for biochemical, ELISA, real-time quantitative PCR, and histopathological analysis. L-egt with or without metformin reduced liver hypertrophy, liver injury, triglycerides, oxidative stress, and inflammation. Also, L-egt normalized mRNA expression of SREBP-1c, fatty acid synthase, nuclear factor kappa B, transforming growth factor \u03b21, nuclear factor erythroid 2\u2013related factor 2, and sirtuin-1 in diabetic rats. Furthermore, co-administration of L-egt with metformin to diabetic rats reduced blood glucose and insulin resistance. These results provide support to the therapeutic benefits of L-egt in the management of liver complications associated with T2D. <\/jats:p>","DOI":"10.1139\/cjpp-2021-0247","type":"journal-article","created":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T17:10:06Z","timestamp":1632849006000},"page":"1137-1147","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":23,"title":["L-ergothioneine and metformin alleviates liver injury in experimental type-2 diabetic rats via reduction of oxidative stress, inflammation, and hypertriglyceridemia"],"prefix":"10.1139","volume":"99","author":[{"given":"Ayobami","family":"Dare","sequence":"first","affiliation":[{"name":"Department of Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban X54001, South Africa."},{"name":"Department of Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban X54001, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahendra L.","family":"Channa","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban X54001, South Africa."},{"name":"Department of Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban X54001, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anand","family":"Nadar","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban X54001, South Africa."},{"name":"Department of Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban X54001, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c170012"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2014.5952"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2015.05.015"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1080\/17474124.2019.1649981"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-13-363"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1615\/IntJMedMushrooms.2021037630"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.24966\/FSN-1076\/100049"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcme.2017.08.012"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21113858"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v10.i1.1"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.3390\/nu12030739"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2016.04.013"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(59)90090-6"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1021\/jf071328f"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1111\/febs.13665"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8765954"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-019-0242-2"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01667"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2020.02.020"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.3390\/nu8080461"},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408624102"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-6915(02)00177-1"},{"key":"refg24\/ref24","doi-asserted-by":"publisher","DOI":"10.2147\/vhrm.s2503"},{"key":"refg25\/ref25","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.13123"},{"key":"refg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2016.01.001"},{"key":"refg27\/ref27","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4545321"},{"key":"refg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajme.2017.09.001"},{"key":"refg29\/ref29","doi-asserted-by":"publisher","DOI":"10.1002\/edm2.116"},{"key":"refg30\/ref30","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3453926"},{"key":"refg31\/ref31","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21020460"},{"key":"refg32\/ref32","doi-asserted-by":"publisher","DOI":"10.7555\/JBR.27.20120077"},{"key":"refg33\/ref33","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-013-0758-5"},{"key":"refg34\/ref34","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.30.1760"},{"key":"refg35\/ref35","doi-asserted-by":"publisher","DOI":"10.2337\/db07-0381"},{"key":"refg36\/ref36","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2016.517"},{"key":"refg37\/ref37","doi-asserted-by":"crossref","unstructured":"Li, H. 2014. Sirtuin 1 (SIRT1) and oxidative stress. In Systems Biology of Free Radicals and Antioxidants. Edited by I. Laher. Springer Berlin Heidelberg, Berlin, Heidelberg.","DOI":"10.1007\/978-3-642-30018-9_17"},{"key":"refg38\/ref38","doi-asserted-by":"publisher","DOI":"10.1615\/IntJMedMushrooms.2020035863"},{"key":"refg39\/ref39","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"refg40\/ref40","doi-asserted-by":"publisher","DOI":"10.1615\/IntJMedMushrooms.2020034560"},{"key":"refg41\/ref41","doi-asserted-by":"publisher","DOI":"10.1186\/s12986-016-0074-1"},{"key":"refg42\/ref42","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/494578"},{"key":"refg43\/ref43","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-011112-140320"},{"key":"refg44\/ref44","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2019.04.006"},{"key":"refg45\/ref45","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD012368.pub2"},{"key":"refg46\/ref46","doi-asserted-by":"publisher","DOI":"10.1590\/0001-3765201920180314"},{"key":"refg47\/ref47","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.3626"},{"key":"refg48\/ref48","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24599"},{"key":"refg49\/ref49","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(85)80613-X"},{"key":"refg50\/ref50","doi-asserted-by":"publisher","DOI":"10.1177\/1091581816653375"},{"key":"refg51\/ref51","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/9547613"},{"key":"refg52\/ref52","doi-asserted-by":"publisher","DOI":"10.1007\/BF00280883"},{"key":"refg53\/ref53","doi-asserted-by":"publisher","DOI":"10.15406\/jnhfe.2014.01.00033"},{"key":"refg54\/ref54","doi-asserted-by":"publisher","DOI":"10.3109\/0886022X.2013.867799"},{"key":"refg55\/ref55","doi-asserted-by":"publisher","DOI":"10.18295\/squmj.2016.16.02.002"},{"key":"refg56\/ref56","doi-asserted-by":"publisher","DOI":"10.1080\/10799893.2020.1726952"},{"key":"refg57\/ref57","doi-asserted-by":"publisher","DOI":"10.1186\/s12944-015-0030-5"},{"key":"refg58\/ref58","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.01.027"},{"key":"refg59\/ref59","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1396"},{"key":"refg60\/ref60","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.01.012"},{"key":"refg61\/ref61","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2019.08.006"},{"key":"refg62\/ref62","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-017-4342-z"},{"key":"refg63\/ref63","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.05.120"},{"key":"refg64\/ref64","doi-asserted-by":"publisher","DOI":"10.3390\/nu13020394"},{"key":"refg65\/ref65","doi-asserted-by":"publisher","DOI":"10.1186\/s13098-019-0431-0"},{"key":"refg66\/ref66","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22729"},{"key":"refg67\/ref67","doi-asserted-by":"publisher","DOI":"10.3803\/EnM.2013.28.1.41"},{"key":"refg68\/ref68","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7290"},{"key":"refg69\/ref69","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/4824371"},{"key":"refg70\/ref70","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2005.05.004"},{"key":"refg71\/ref71","first-page":"A423","volume":"33","author":"Srivastava R.A.K.","year":"2013","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"refg72\/ref72","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-20021-z"},{"key":"refg73\/ref73","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2016.02.023"},{"key":"refg74\/ref74","first-page":"123","volume":"38","author":"Thambiah S.C.","year":"2016","journal-title":"Malays. J. Pathol."},{"key":"refg75\/ref75","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7342"},{"key":"refg76\/ref76","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2018.01.034"},{"key":"refg77\/ref77","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.119.13929"},{"key":"refg78\/ref78","doi-asserted-by":"publisher","DOI":"10.1016\/S1734-1140(12)70739-9"},{"key":"refg79\/ref79","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.153139"},{"key":"refg80\/ref80","doi-asserted-by":"publisher","DOI":"10.1007\/s40139-015-0093-z"},{"key":"refg81\/ref81","doi-asserted-by":"publisher","DOI":"10.14218\/JCTH.2014.00037"},{"key":"refg82\/ref82","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b02083"},{"key":"refg83\/ref83","doi-asserted-by":"publisher","DOI":"10.1007\/s11684-015-0384-0"},{"key":"refg84\/ref84","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2014.132"}],"container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/cjpp-2021-0247","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/cjpp-2021-0247","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T14:13:41Z","timestamp":1751465621000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/cjpp-2021-0247"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":84,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1139\/cjpp-2021-0247"],"URL":"https:\/\/doi.org\/10.1139\/cjpp-2021-0247","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11]]},"assertion":[{"value":"2021-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}