{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T23:32:03Z","timestamp":1769038323958,"version":"3.49.0"},"reference-count":55,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:p> Sarco(endo)plasmic reticulum calcium (Ca<jats:sup>2+<\/jats:sup>) ATPase (SERCA) transports Ca<jats:sup>2+<\/jats:sup> in muscle. Impaired SERCA activity may contribute to diabetic myopathy. Sirtuin (SIRT) 3 regulates muscle metabolism and function; however, it is unknown if SIRT3 regulates muscle SERCA activity or acetylation. We determined if SIRT3 overexpression enhances SERCA activity in mouse gastrocnemius muscle and if SIRT3 overexpression preserves gastrocnemius SERCA activity in a model of type 2 diabetes, induced by high fat \u2013 high sucrose (HFHS) feeding. We also determined if the acetylation status of SERCA proteins in mouse gastrocnemius is altered by SIRT3 overexpression or HFHS feeding. Wild-type (WT) and SIRT3 transgenic (SIRT3<jats:sub>TG<\/jats:sub>) mice, overexpressing SIRT3 in skeletal muscle, were fed a standard or HFHS diet for 4 months. SIRT3<jats:sub>TG<\/jats:sub> and WT mice developed obesity and glucose intolerance after 4 months of HFHS feeding. SERCA V<jats:sub>max<\/jats:sub> was higher in gastrocnemius of SIRT3<jats:sub>TG<\/jats:sub> mice compared with WT mice. HFHS-fed mice had lower SERCA1a protein levels and lower SERCA V<jats:sub>max<\/jats:sub> in their gastrocnemius than control-fed mice. The decrease in SERCA V<jats:sub>max<\/jats:sub> in gastrocnemius muscle due to HFHS feeding was attenuated by SIRT3 overexpression in HFHS-fed SIRT3<jats:sub>TG<\/jats:sub> mice. SERCA1a and SERCA2a acetylation in mouse gastrocnemius was not altered by genotype or diet. These findings suggest SIRT3 overexpression improves SERCA function in mouse skeletal muscle. <\/jats:p>","DOI":"10.1139\/cjpp-2021-0587","type":"journal-article","created":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T21:35:17Z","timestamp":1635197717000},"page":"361-370","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Sirtuin 3 overexpression preserves maximal sarco(endo)plasmic reticulum calcium ATPase activity in the skeletal muscle of mice subjected to high fat \u2013 high sucrose feeding"],"prefix":"10.1139","volume":"100","author":[{"given":"Christopher J.","family":"Oldfield","sequence":"first","affiliation":[{"name":"Faculty of Kinesiology and Recreation Management, University of Manitoba, Winnipeg, MN R3T 2N2, Canada."},{"name":"Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, Winnipeg, MN R2H 2A6, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teri L.","family":"Moffatt","sequence":"additional","affiliation":[{"name":"Faculty of Kinesiology and Recreation Management, University of Manitoba, Winnipeg, MN R3T 2N2, Canada."},{"name":"Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, Winnipeg, MN R2H 2A6, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vernon W.","family":"Dolinsky","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MN R3E 0W2, Canada."},{"name":"Diabetes Research Envisioned and Accomplished in Manitoba (DREAM), Theme of the Children\u2019s Hospital Research Institute of Manitoba, Winnipeg, MN R3E 3P4, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd 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