{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T14:15:32Z","timestamp":1774275332346,"version":"3.50.1"},"reference-count":32,"publisher":"Canadian Science Publishing","issue":"6","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2022,6,1]]},"abstract":"<jats:p> Transcranial Doppler ultrasonography is used to study intracranial blood flow changes associated with migraine in humans, but whether this method is helpful in preclinical settings is yet unknown. To identify changes in rat intracranial blood flow specific to trigeminovascular activation\u2014a key process in migraine pathophysiology\u2014we measured Doppler indices in the middle cerebral artery and basilar artery before, during, and after dural or somatosensory electrical stimulation. Hemodynamic changes specific to dural stimulation were tested further in separate experiments. After baseline recordings, the animals received cumulative infusions of valproate (100\u2009mg\/kg, trice), sumatriptan (0.3, 1, and 3\u00a0mg\/kg), or saline, and dural stimulation with measurement of Doppler indices was repeated every 10\u2009min for 1\u2009h. Several parameters of blood flow in the rat middle cerebral artery underwent alterations specific to trigeminovascular activation. These changes, however, were insensitive to valproate and sumatriptan and diminished over time. These findings question the reliability of blood flow velocity variations in large intracranial vessels as biological markers of migraine-related processes and do not support the idea of using transcranial Doppler ultrasonography for preclinical screening of antimigraine treatments, at least in the model of acute trigeminovascular activation in rats. <\/jats:p>","DOI":"10.1139\/cjpp-2021-0626","type":"journal-article","created":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T21:34:41Z","timestamp":1649799281000},"page":"553-561","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["The implementation of transcranial Doppler ultrasonography for preclinical study of migraine"],"prefix":"10.1139","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4189-7910","authenticated-orcid":false,"given":"Antonina","family":"Dolgorukova","sequence":"first","affiliation":[{"name":"Department of Neuropharmacology, Valdman Institute of Pharmacology, Pavlov First Saint Petersburg State Medical University","place":["Saint Petersburg, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasiia V.","family":"Potapenko","sequence":"additional","affiliation":[{"name":"Department of Neuropharmacology, Valdman Institute of Pharmacology, Pavlov First Saint Petersburg State Medical University","place":["Saint Petersburg, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna A.","family":"Murzina","sequence":"additional","affiliation":[{"name":"Department of Neuropharmacology, Valdman Institute of Pharmacology, Pavlov First Saint Petersburg State Medical University","place":["Saint Petersburg, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga A.","family":"Lyubashina","sequence":"additional","affiliation":[{"name":"Department of Neuropharmacology, Valdman Institute of Pharmacology, Pavlov First Saint Petersburg State Medical University","place":["Saint Petersburg, Russia"]},{"name":"Laboratory of Cortico-Visceral Physiology, Pavlov Institute of Physiology of the Russian Academy of Sciences","place":["Saint Petersburg, Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexey","family":"Y. 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