{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:41:32Z","timestamp":1787017292398,"version":"build-2736575974"},"reference-count":42,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2022,8,1]]},"abstract":"<jats:p>\n                    Carvacrol (CRV) is the main compound of essential oils extracted primarily from Thymus and Origanum species. Its various biological activities were confirmed: antioxidant, anti-inflammatory, antibacterial, antifungal, anti-tumour, antinematodal, and vasorelaxant action. Although vasodilation mediated by CRV was previously described, the exact mechanism of its action has not yet been established. Hence, the aim of this study was to investigate CRV vasoactivity on human umbilical arteries (HUA) and the different pathways involved in its mechanism of action using the tissue bath methodology. CRV caused a significant decrease in vascular tension of 5-HT-pre-contracted umbilical arteries, with EC\n                    <jats:sub>50<\/jats:sub>\n                    of 442.13\u00a0\u00b1\u00a033.8\u00a0\u00b5mol\/L (mean\u00a0\u00b1\u00a0standard error of the mean\u2014SEM). At 300\u00a0\u00b5mol\/L, CRV shifted downward the 5-HT concentration\u2013response curve with a statistical significance of p\u00a0&lt;\u00a00.001 obtained for the four highest concentrations. At a concentration of 1\u00a0mmol\/L, CRV completely abolished BaCl\n                    <jats:sub>2<\/jats:sub>\n                    -induced contraction in Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -free Krebs\u2013Ringer bicarbonate solution and the BAY K 8644-induced contraction in Krebs\u2013Ringer bicarbonate solution ( p\u00a0&lt;\u00a00.001). Isopentenyl pyrophosphate, the antagonist of TRPV\n                    <jats:sub>3<\/jats:sub>\n                    channel, was able to decrease the efficacy of CRV ( p\u00a0&lt;\u00a00.001). The blocking of L-type Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels on smooth muscle cells is the most probable mechanism of CRV-induced vasorelaxation. However, the role of TRPV\n                    <jats:sub>3<\/jats:sub>\n                    channels in CRV-induced vasodilation of HUA cannot be excluded either.\n                  <\/jats:p>","DOI":"10.1139\/cjpp-2021-0736","type":"journal-article","created":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T17:36:02Z","timestamp":1651685762000},"page":"755-762","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery"],"prefix":"10.1139","volume":"100","author":[{"given":"\u0110or\u0111e","family":"\u0110ukanovi\u0107","sequence":"first","affiliation":[{"name":"Centre for Biomedical Research, Faculty of Medicine, University of Banja Luka","place":["Banja Luka, Bosnia and Herzegovina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milica Gaji\u0107","family":"Boji\u0107","sequence":"additional","affiliation":[{"name":"Centre for Biomedical Research, Faculty of Medicine, University of Banja Luka","place":["Banja Luka, Bosnia and Herzegovina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Marinkovi\u0107","sequence":"additional","affiliation":[{"name":"Centre for Biomedical Research, Faculty of Medicine, University of Banja Luka","place":["Banja Luka, Bosnia and Herzegovina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa\u0161a","family":"Trailovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Belgrade","place":["Belgrade, Serbia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milo\u0161 P.","family":"Stojiljkovi\u0107","sequence":"additional","affiliation":[{"name":"Centre for Biomedical Research, Faculty of Medicine, University of Banja Luka","place":["Banja Luka, Bosnia and Herzegovina"]},{"name":"Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Banja Luka","place":["Banja Luka, Bosnia and Herzegovina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranko","family":"\u0160krbi\u0107","sequence":"additional","affiliation":[{"name":"Centre for Biomedical Research, Faculty of Medicine, University of Banja Luka","place":["Banja Luka, Bosnia and Herzegovina"]},{"name":"Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Banja Luka","place":["Banja Luka, Bosnia and Herzegovina"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-1050-8"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1972.222.2.345"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2011.01.044"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(95)90468-9"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9378(99)70268-1"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23061430"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2016.11.008"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2015.02.016"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2004.05.002"},{"issue":"3","key":"refg10\/ref10","first-page":"134","volume":"51","author":"\u0110ukanovi\u0107 \u0110.","year":"2020","journal-title":"Scr. Med."},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1053\/j.semperi.2009.02.010"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1124\/mol.109.060715"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25112681"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.5.1788"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1159\/000174250"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1097\/01.hjh.0000234115.40648.88"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M900255-JLR200"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-011-1043-7"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1973.tb16050.x"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-947255"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68397-9"},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2001.01428.x"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1111\/jog.13667"},{"key":"refg24\/ref24","doi-asserted-by":"publisher","DOI":"10.3390\/cells9091956"},{"key":"refg25\/ref25","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702647"},{"key":"refg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.059865"},{"key":"refg27\/ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.ando.2020.03.012"},{"key":"refg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2016.04.004"},{"key":"refg29\/ref29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.2009.00768.x"},{"key":"refg30\/ref30","doi-asserted-by":"publisher","DOI":"10.3390\/molecules18033072"},{"key":"refg31\/ref31","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-018-2201-5"},{"key":"refg32\/ref32","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00140.2015"},{"key":"refg33\/ref33","doi-asserted-by":"publisher","DOI":"10.1291\/hypres.25.589"},{"key":"refg34\/ref34","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-971222"},{"key":"refg35\/ref35","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6103"},{"key":"refg36\/ref36","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60279-6"},{"key":"refg37\/ref37","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.12585"},{"key":"refg38\/ref38","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-015-4508-x"},{"key":"refg39\/ref39","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707245"},{"key":"refg40\/ref40","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(88)90408-6"},{"key":"refg41\/ref41","doi-asserted-by":"publisher","DOI":"10.1038\/nn1692"},{"key":"refg42\/ref42","doi-asserted-by":"publisher","DOI":"10.1097\/00005237-200407000-00006"}],"container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/cjpp-2021-0736","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/cjpp-2021-0736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T12:22:22Z","timestamp":1754482942000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/cjpp-2021-0736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,1]]},"references-count":42,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8,1]]}},"alternative-id":["10.1139\/cjpp-2021-0736"],"URL":"https:\/\/doi.org\/10.1139\/cjpp-2021-0736","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,1]]},"assertion":[{"value":"2022-05-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}