{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:22:14Z","timestamp":1792624934158,"version":"4.5.0"},"reference-count":55,"publisher":"Canadian Science Publishing","issue":"7","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2022,7,1]]},"abstract":"<jats:p> Mesenchymal stem cell-derived conditioned medium (MSC-CM) improves cardiac function, which is partly attributed to the released paracrine factors. Since such cardioprotection is moderate and transient, it is essential that MSC-CM's effective components are optimized to alleviate myocardial injury. To optimize MSC-CM, MSCs were treated with or without lipopolysaccharides (LPSs) for 48 h (serum-free), and the supernatant was collected. Then, LPS-CM (MSC stimulated by LPS) was further treated with LPS remover (LPS Re-CM) or was concentrated with a 10\u2009kDa cutoff filter (10 kDa-CM). Enzyme-linked immunosorbent assay showed that all the pretreatments increased the levels of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and insulin growth factor (IGF) except LPS Re-CM; 10 kDa-CM was superior to the other CMs. Cell Counting Kit-8 displayed that the viability of injured H9c2 cells was enhanced with the increase in the MSC-CM concentration. We also found that the 10 kDa-CM significantly alleviated H9c2 hypoxia\/reoxygenation (H\/R) injury, as evidenced by the increased Bcl-2\/Bax ratio, and decreased the levels of lactate dehydrogenase and cardiac troponin. Transmission electron microscopy (TEM), TdT-mediated dUTP nick-end labelling (TUNEL), and hematoxylin and eosin staining (H&amp;E) confirmed that 10 kDa-CM inhibited H\/R-induced H9c2 morphological changes. Proteomic analysis identified 41 differentially expressed proteins in 10 kDa-CM, among which anti-inflammation, proangiogenesis, and antiapoptosis were related to cardiac protection. This study indicates that 10 kDa-CM protects H9c2 cardiomyocytes from H\/R injury by preserving most of the protective factors, such as VEGF, HGF, and IGF, in MSC-CM. <\/jats:p>","DOI":"10.1139\/cjpp-2021-0745","type":"journal-article","created":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T18:30:28Z","timestamp":1652121028000},"page":"651-664","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Lipopolysaccharide-pretreated mesenchymal stem cell-conditioned medium optimized with 10\u2009kDa filter attenuates the injury of H9c2 cardiomyocytes in a model of hypoxia\/reoxygenation"],"prefix":"10.1139","volume":"100","author":[{"given":"Dan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Ordos Central Hospital","place":["Ordos, 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