{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:57:50Z","timestamp":1791824270261,"version":"4.3.4"},"reference-count":67,"publisher":"Canadian Science Publishing","issue":"12","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2022,12,1]]},"abstract":"<jats:p> Aberrant activation of Wnt\/\u03b2-catenin induces renal dysfunction by initiating pro-apoptotic cascades, fibrosis, oxidative and inflammatory burden. This study tested the therapeutic effects of Wnt\/\u03b2-catenin inhibitor pyrvinium against cisplatin-induced acute kidney injury (AKI) in rats. Cisplatin was administered at a single dose of 5\u00a0mg\/kg (i.p.) and renal cisplatin accumulation and uptake in cortical slices were determined after the fifth day by atomic absorption spectroscopy. Levels of pro-inflammatory cytokines were checked by ELISA, and organic cation transporter-2 (OCT-2) transcription and expression in renal tissue were evaluated by RT-PCR and immunohistochemical technique. Cisplatin administration produced renal dysfunction manifested as increase in serum creatinine, blood urea nitrogen, proteinuria, reduced clearance and electrolyte imbalance. Oxidative stress indices, pro-inflammatory cytokines, fibronectin, and caspase-3 activity were elevated in cisplatin-challenged rats. Moreover, increased renal OCT-2 transcription and immunostaining were detected in cisplatin kidneys which resulted in platinum accumulation. Additional docking studies depicted strong interaction between the \u03b2-catenin and OCT-2 protein. These manifestations induced mitochondrial dysfunction, histological damage and fibrosis. Notably, Wnt\/\u03b2-catenin inhibitor pyrvinium (60\u00a0\u00b5g\/kg; p.o.) treatment reduced the renal OCT-2 gene transcription causing a decline in platinum levels. Thus, the present study concludes that Wnt\/\u03b2-catenin inhibition attenuates cisplatin-induced AKI in rats, partly by down-regulating OCT-2 expression. <\/jats:p>","DOI":"10.1139\/cjpp-2022-0165","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T21:34:10Z","timestamp":1664314450000},"page":"1115-1134","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Wnt\/\u03b2-catenin inhibitor pyrivinium attenuates cisplatin-induced acute kidney injury by possibly reducing platinum uptake and accumulation mediated by reduced organic cation transporter-2 expressions"],"prefix":"10.1139","volume":"100","author":[{"given":"Sneha","family":"Pandey","sequence":"first","affiliation":[{"name":"Department of Pharmacology","place":["India"]},{"name":"Indo-Soviet College of Pharmacy","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirti","family":"Gupta","sequence":"additional","affiliation":[{"name":"Maharishi Markandeshwar College of Pharmacy","place":["India"]},{"name":"Maharishi Markandeshwar deemed to be University","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Newly","family":"Bagang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy","place":["Namsai, India"]},{"name":"Arunachal University of Studies","place":["Namsai, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4032-9049","authenticated-orcid":false,"given":"Gaaminepreet","family":"Singh","sequence":"additional","affiliation":[{"name":"Chitkara College of Pharmacy","place":["India"]},{"name":"Chitkara University","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakshi","family":"Rajput","sequence":"additional","affiliation":[{"name":"Sunder deep Pharmacy College","place":["Ghaziabad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-091302-2.50032-3"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03751.x"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1267\/ahc.16025"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2019.03.031"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0270-9295(03)00089-5"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2003.11.003"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2019.05.029"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490290309"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5698931"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(72)90335-1"},{"key":"refg11\/ref11","first-page":"72","volume":"26","author":"Capizzi RL.","year":"1999","journal-title":"Semin. 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