{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:43:11Z","timestamp":1761514991544,"version":"3.43.0"},"reference-count":32,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2023,5,1]]},"abstract":"<jats:p> Molecular modification of compounds remains important strategy towards the discovery of new drugs.\u00a0In this sense, this study presents a new\u00a0pyrazole derivative\u00a05-(1-(2-fluorophenyl)-1 H-pyrazol-4-yl)-1 H-tetrazole (LQFM039) and evaluated the anti-inflammatory, analgesic,\u00a0and\u00a0vasorelaxant\u00a0effects of this compound as well the mechanisms of action involved in the pharmacological effects. For this, mice were orally treated with LQFM039 (17.5, 35, or 70\u2009mg\/kg) prior acetic acid-induced abdominal writhing, formalin, tail flick, and carrageenan-induced paw edema protocols. In addition,\u00a0vascular reactivity protocols were made with aortic rings contraction with phenylephrine and stimulated with graded concentrations of LQFM039. Abdominal writhing and licking time in both neurogenic and inflammatory phases of formalin were reduced with LQFM039 without altering\u00a0latency to nociceptive response\u00a0in the tail flick test. Carrageenan-induced paw edema showed that LQFM039 reduces edema and cell migration. In addition, the mechanism of action of LQFM039 involves NO\/cGMP pathway and calcium channels, since this new pyrazole derivate elicited\u00a0concentration-dependent relaxation attenuated by\u00a0N\u03c9-nitro-l-arginine methyl ester\u00a0and\u00a01 H-[1,2,4] oxadiazolo [4,3-alpha]quinoxalin-1-one, and blockade of CaCl<jats:sub>2<\/jats:sub>-induced contraction. Altogether, our finding suggests anti-inflammatory, antinociceptive, and vasorelaxant\u00a0effect\u00a0of this new pyrazole derivative with involvement of NO\/cGMP pathway and calcium channels. <\/jats:p>","DOI":"10.1139\/cjpp-2022-0428","type":"journal-article","created":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T07:00:10Z","timestamp":1677826810000},"page":"216-225","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Anti-inflammatory, antinociceptive, and vasorelaxant effects of a new pyrazole compound 5-(1-(2-fluorophenyl)-1<i>H<\/i>-pyrazol-4-yl)-1<i>H<\/i>-tetrazole: role of NO\/cGMP pathway and calcium channels"],"prefix":"10.1139","volume":"101","author":[{"given":"Lanussy P.","family":"de Oliveira","sequence":"first","affiliation":[{"name":"Department of Pharmacology, ICB, Federal University of Goi\u00e1s, Campus Samambaia","place":["Goi\u00e2nia, 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