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J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:p>Ischemia\u2013reperfusion (IR) injury of vascular grafts used in coronary artery bypass surgery can compromise outcomes. This study examined whether GYY4137, a slow-release hydrogen sulfide donor, protects against IR-induced vascular damage in rat thoracic aorta. Twenty male Sprague-Dawley rats (21\u201322 months old) were randomly assigned to Control, IR, IR\u00a0+\u00a0GYY4137 (100\u00a0\u00b5M), and IR\u00a0+\u00a0GYY4137 (300\u00a0\u00b5M) groups. To induce IR injury, aortic rings were incubated in nitrogen-gassed saline at 4 \u00b0C for 24 h, then exposed to sodium hypochlorite (200\u00a0\u00b5M) at 37 \u00b0C for 30 min. Vascular function was evaluated in organ baths; malondialdehyde, glutathione, and caspase-3 were quantified. IR injury markedly impaired contraction and endothelium-dependent relaxation, which GYY4137 did not restore. In contrast, IR increased malondialdehyde ( p\u00a0=\u00a00.02), which fell to near-control with both 100\u00a0\u00b5M ( p\u00a0=\u00a00.008) and 300\u00a0\u00b5M GYY4137 ( p\u00a0=\u00a00.012). IR lowered glutathione ( p\u00a0=\u00a00.012) was restored by 300\u00a0\u00b5M GYY4137 ( p\u00a0=\u00a00.004). Likewise, caspase-3 rose ( p\u00a0=\u00a00.02) but returned to near-control with both 100\u00a0\u00b5M ( p\u00a0=\u00a00.004) and 300\u00a0\u00b5M GYY4137 ( p\u00a0=\u00a00.02). In conclusion, GYY4137 reduces oxidative stress and apoptosis markers without improving vascular dysfunction in an IR model of rat thoracic aorta. This disparity underscores the complex pathophysiology of vascular IR injury, where cellular-level protection does not readily translate to functional recovery.<\/jats:p>","DOI":"10.1139\/cjpp-2025-0168","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T21:33:41Z","timestamp":1761082421000},"page":"1-10","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of slow-release hydrogen sulfide donor GYY4137 on vascular smooth muscle and endothelial response in an in vitro ischemia\u2013reperfusion model of rat thoracic aorta"],"prefix":"10.1139","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3299-9491","authenticated-orcid":false,"given":"Ariyan","family":"Teimoori","sequence":"first","affiliation":[{"name":"Ba\u015fkent University","place":["Ankara, 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