{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:08:34Z","timestamp":1789470514548,"version":"build-2803163510"},"reference-count":37,"publisher":"Canadian Science Publishing","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:p>\n                    Endothelin-1 (ET-1) antagonists are increasingly being approved for new treatments for cardiovascular disease, where elevated ET-1 levels contribute to increased vasoconstriction. Further therapeutic targets, including coronary artery disease, are under investigation. The Olink Explore 3072 Proximity Extension Assay platform enables multiplexed high-throughput measurement of ~3000 plasma proteins, from minimal (\u22646\u2009\u00b5L) sample volumes. However, it is not known if the two oligonucleotide\u2013tagged antibodies raised against preproET-1\n                    <jats:sub>1-212<\/jats:sub>\n                    , used in this Olink assay, specifically measure biologically active ET-1 or the other inactive EDN1-encoded peptides, also secreted by human endothelial cells. Paired plasma samples from 29 patients with coronary artery disease were obtained, using a specialised intra-coronary sampling catheter, designed to obtain site specific biochemical information from within coronary arteries. We compared ET-1 concentrations measured with an ET-1 specific ELISA, demonstrated to have no cross-reactivity with other EDN1-encoded peptides versus values obtained using Olink Explore platform. Olink-measured ET-1 correlated significantly with ELISA-derived ET-1 levels ( r\u00a0=\u00a00.53, p\u00a0=\u00a00.003), and Olink values predicted ELISA results. Olink ET-1 concentrations also correlated with ET\n                    <jats:sub>B<\/jats:sub>\n                    receptor levels ( r\u00a0=\u00a00.40, p\u00a0&lt;\u00a00.05). These findings indicate that the Olink Explore platform can detect relative changes in biologically active ET-1, supporting its use as a biomarker tool in clinical and translational studies.\n                  <\/jats:p>","DOI":"10.1139\/cjpp-2026-0112","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T21:35:56Z","timestamp":1787348156000},"page":"1-9","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of endothelin-1 levels in plasma from human coronary arteries measured by enzyme linked immunosorbent assay and Olink high-throughput proteomics platform"],"prefix":"10.1139","volume":"104","author":[{"given":"Majid","family":"Anwar","sequence":"first","affiliation":[{"name":"Royal Papworth Hospital","place":["Cambridge, UK"]},{"name":"University of Cambridge","place":["Cambridge, UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhoda 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