{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T19:09:54Z","timestamp":1760382594845},"reference-count":17,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[1995,8,1]],"date-time":"1995-08-01T00:00:00Z","timestamp":807235200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genome"],"published-print":{"date-parts":[[1995,8,1]]},"abstract":"<jats:p> Immature embryos of Triticum tauschii cultured on L2 medium with 30\u2002g\u2219L<jats:sup>\u22121<\/jats:sup>maltose gave rise to compact and highly structured callus that readily regenerated shoots when transferred to MS medium supplemented with zeatin and indole-3-acetic acid. Two cell suspensions were initiated from the callus induced on L2 medium. An analysis of chromosome number in these cell suspensions after 3 months of culture showed that 95 and 75% of the cells had a normal complement. After 5 months, in both lines the majority of cells had chromosome complements of 2n\u2002=\u200213 and many of these aneuploid cells possessed a dicentric chromosome. C-banding indicated that the dicentric was often formed from chromosomes 2D and 5D. Fifty-six plants regenerated from callus were grown to maturity. Variation was observed in tiller number, flowering time, and seed-set; in two cases, the variation in early flowering was shown to be heritable. All the regenerants analysed had normal chromosome counts (2n\u2002=\u20022x\u2002=\u200214) and meiotic analyses of 35 plants revealed no obvious structural rearrangements.Key words: immature embryo culture, regeneration, chromosome, cell suspension, Triticum tauschii. <\/jats:p>","DOI":"10.1139\/g95-092","type":"journal-article","created":{"date-parts":[[2008,5,9]],"date-time":"2008-05-09T19:06:01Z","timestamp":1210359961000},"page":"737-742","source":"Crossref","is-referenced-by-count":5,"title":["Chromosome 5D instability in cell lines of <i>Triticum tauschii<\/i> and morphological variation in regenerated plants"],"prefix":"10.1139","volume":"38","author":[{"given":"M. O.","family":"Winfield","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Karp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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