{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T00:18:05Z","timestamp":1781309885110,"version":"3.54.1"},"reference-count":62,"publisher":"Canadian Science Publishing","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Genome"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:p> Species in the cycad genus Encephalartos are listed in CITES Appendix I and as Threatened or Protected Species in terms of South Africa\u2019s National Environmental Management: Biodiversity Act (NEM:BA) of 2004. Despite regulations, illegal plant harvesting for medicinal trade has continued in South Africa and resulted in declines in cycad populations and even complete loss of sub-populations. Encephalartos is traded at traditional medicine markets in South Africa in the form of bark strips and stem sections; thus, determining the species traded presents a major challenge due to a lack of characteristic plant parts. Here, a case study is presented on the use of DNA barcoding to identify cycads sold at the Faraday and Warwick traditional medicine markets in Johannesburg and Durban, respectively. Market samples were sequenced for the core DNA barcodes (rbcLa and matK) as well as two additional regions: nrITS and trnH-psbA. The barcoding database for cycads at the University of Johannesburg was utilized to assign query samples to known species. Three approaches were followed: tree-based, similarity-based, and character-based (BRONX) methods. Market samples identified were Encephalartos ferox (Near Threatened), Encephalartos lebomboensis (Endangered), Encephalartos natalensis (Near Threatened), Encephalartos senticosus (Vulnerable), and Encephalartos villosus (Least Concern). Results from this study are crucial for making appropriate assessments and decisions on how to manage these markets. <\/jats:p>","DOI":"10.1139\/gen-2016-0032","type":"journal-article","created":{"date-parts":[[2016,7,14]],"date-time":"2016-07-14T04:02:34Z","timestamp":1468468954000},"page":"771-781","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":19,"title":["Exposing the illegal trade in cycad species (Cycadophyta: <i>Encephalartos<\/i>) at two traditional medicine markets in South Africa using DNA barcoding"],"prefix":"10.1139","volume":"59","author":[{"given":"J.","family":"Williamson","sequence":"first","affiliation":[{"name":"The African Centre for DNA Barcoding, Department of Botany & Plant Biotechnology, University of Johannesburg, APK Campus, P.O. Box 524, Auckland Park, 2006, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Maurin","sequence":"additional","affiliation":[{"name":"The African Centre for DNA Barcoding, Department of Botany & Plant Biotechnology, University of Johannesburg, APK Campus, P.O. Box 524, Auckland Park, 2006, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.N.S.","family":"Shiba","sequence":"additional","affiliation":[{"name":"The African Centre for DNA Barcoding, Department of Botany & Plant Biotechnology, University of Johannesburg, APK Campus, P.O. Box 524, Auckland Park, 2006, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"van der Bank","sequence":"additional","affiliation":[{"name":"The African Centre for DNA Barcoding, Department of Zoology, University of Johannesburg, APK Campus, P.O. Box 524, Auckland Park, 2006, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Pfab","sequence":"additional","affiliation":[{"name":"South African National Biodiversity Institute, Pretoria National Botanical Garden, P\/Bag X101, Silverton, 0184, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Pilusa","sequence":"additional","affiliation":[{"name":"The African Centre for DNA Barcoding, Department of Botany & Plant Biotechnology, University of Johannesburg, APK Campus, P.O. Box 524, Auckland Park, 2006, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.M.","family":"Kabongo","sequence":"additional","affiliation":[{"name":"The African Centre for DNA Barcoding, Department of Botany & Plant Biotechnology, University of Johannesburg, APK Campus, P.O. Box 524, Auckland Park, 2006, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"van der Bank","sequence":"additional","affiliation":[{"name":"The African Centre for DNA Barcoding, Department of Botany & Plant Biotechnology, University of Johannesburg, APK Campus, P.O. 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Marketing of indigenous medicinal plants in South Africa: a case study in KwaZulu-Natal. Food and Agriculture Organization of the United Nations, Rome."},{"key":"refg39\/ref39","unstructured":"Mander, M., Ntuli, L., Diederichs, N., and Mavundla, K. 2007. Economics of the traditional medicine trade in South Africa. In South African Health Review 2007. Edited by S. Harrison, R. Bhana, and A. Ntuli. Health Systems Trust, Durban."},{"key":"refg41\/ref41","unstructured":"NEM:BA. 2004. National Environmental Management: Biodiversity Act (No. 10 of 2004). 471 Government Gazette No. 32090, 3 April 2009. Department of Environmental Affairs and Tourism, Pretoria."},{"key":"refg42\/ref42","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-11-222"},{"key":"refg43\/ref43","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-0031.2010.00321.x"},{"key":"refg44\/ref44","unstructured":"Norstog, K.J., and Nicholls, T.J. 1997. The biology of the cycads. 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