{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:37:52Z","timestamp":1790354272486,"version":"4.1.0"},"reference-count":45,"publisher":"Canadian Science Publishing","issue":"2","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Genome"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:p> For commercial oyster aquaculture, triploidy has significant advantages. To produce triploids, the principal technology uses diploid \u00d7 tetraploid crosses. The development of tetraploid brood stock for this purpose has been successful, but as more is understood about tetraploids, it seems clear that chromosome instability is a principal feature in oysters. This paper is a continuation of work to investigate chromosome instability in polyploid Crassostrea virginica. We established families between tetraploids\u2014apparently stable (non-mosaic) and unstable (mosaic)\u2014and normal reference diploids, creating triploid groups, as well as tetraploids between mosaic and non-mosaic tetraploids. Chromosome loss was about the same for triploid juveniles produced from either mosaic or non-mosaic tetraploids or from either male or female tetraploids. However, there was a statistically significant difference in chromosome loss in tetraploid juveniles produced from mosaic versus non-mosaic parents, with mosaics producing more unstable progeny. These results confirm that chromosome instability, as manifested in mosaic tetraploids, is of little concern for producing triploids, but it is clearly problematic for tetraploid breeding. Concordance between the results from cytogenetics and flow cytometry was also tested for the first time in oysters, by assessing the ploidy of individuals using both techniques. Results between the two were non-concordant. <\/jats:p>","DOI":"10.1139\/gen-2017-0173","type":"journal-article","created":{"date-parts":[[2017,11,30]],"date-time":"2017-11-30T18:39:51Z","timestamp":1512067191000},"page":"79-89","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":21,"title":["Mitotic instability in triploid and tetraploid one-year-old eastern oyster, <i>Crassostrea virginica<\/i>, assessed by cytogenetic and flow cytometry techniques"],"prefix":"10.1139","volume":"61","author":[{"given":"Joana Teixeira","family":"de Sousa","sequence":"first","affiliation":[{"name":"Aquaculture Genetics and Breeding Technology Center. Virginia Institute of Marine Science, Gloucester Point, VA 23062, USA."},{"name":"Aquaculture Genetics and Breeding Technology Center. Virginia Institute of Marine Science, Gloucester Point, VA 23062, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Standish K.","family":"Allen","sequence":"additional","affiliation":[{"name":"Aquaculture Genetics and Breeding Technology Center. Virginia Institute of Marine Science, Gloucester Point, VA 23062, USA."},{"name":"Aquaculture Genetics and Breeding Technology Center. Virginia Institute of Marine Science, Gloucester Point, VA 23062, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brittany M.","family":"Wolfe","sequence":"additional","affiliation":[{"name":"Aquaculture Genetics and Breeding Technology Center. Virginia Institute of Marine Science, Gloucester Point, VA 23062, USA."},{"name":"Aquaculture Genetics and Breeding Technology Center. Virginia Institute of Marine Science, Gloucester Point, VA 23062, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica Moss","family":"Small","sequence":"additional","affiliation":[{"name":"Aquaculture Genetics and Breeding Technology Center. Virginia Institute of Marine Science, Gloucester Point, VA 23062, USA."},{"name":"Aquaculture Genetics and Breeding Technology Center. Virginia Institute of Marine Science, Gloucester Point, VA 23062, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(03)00190-0"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(83)90412-X"},{"key":"refg5\/ref5","first-page":"58","volume":"31","author":"Allen S.K.","year":"1988","journal-title":"Oceanica"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(92)90170-P"},{"key":"refg3\/ref3","first-page":"514","volume":"15","author":"Allen S.K.","year":"1996","journal-title":"Fact or artifact. J. Shellfish Res."},{"key":"refg6\/ref6","doi-asserted-by":"publisher","unstructured":"Al-Mozain, N., Mashi, A., Belgaumi, A., Al-Sweedan, S., Rawas, F., Ramadan, S., et al. 2015. Discrepancies between DNA index by flow cytometry and cytogenetic studies in childhood? Lymphoblastic leukemia. J. Clin. Exp. Pathol. 10.4172\/2161-0681.S1.016.","DOI":"10.4172\/2161-0681.S1.016"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.58.032806.103835"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2009.12.003"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1112041109"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1711"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1139\/gen-2015-0222"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.cpb.2014.09.002"},{"key":"refg55\/ref55","first-page":"159","author":"Erskine A.J.","year":"2003","journal-title":"thesis, College of William and Mary, Virginia Institute of Marine Science, Gloucester Point, VA"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.2307\/1542093"},{"key":"refg19\/ref19","first-page":"733","volume":"43","author":"Ford J.H.","year":"1988","journal-title":"Am. J. Hum. Genet."},{"key":"refg21\/ref21","first-page":"42","volume":"3","author":"Guo X.","year":"1994","journal-title":"Mol. Mar. Biol. Biotechnol."},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(95)01243-5"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1533\/9781845696474.1.165"},{"key":"refg24\/ref24","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2109.1999.00296.x"},{"key":"refg25\/ref25","doi-asserted-by":"publisher","DOI":"10.1508\/cytologia.64.247"},{"key":"refg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1016\/S0990-7440(01)01119-6"},{"key":"refg27\/ref27","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(00)00488-9"},{"key":"refg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2014.05.015"},{"key":"refg29\/ref29","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(90)90024-X"},{"key":"refg30\/ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2005.02.003"},{"key":"refg31\/ref31","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(01)00861-4"},{"issue":"2","key":"refg32\/ref32","first-page":"979","volume":"19","author":"Park G.M.","year":"2000","journal-title":"J. Shellfish Res."},{"key":"refg33\/ref33","doi-asserted-by":"publisher","DOI":"10.1093\/mollus\/eym025"},{"key":"refg34\/ref34","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2009.04.036"},{"key":"refg35\/ref35","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)61723-9"},{"key":"refg36\/ref36","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2011(94)90025-6"},{"key":"refg38\/ref38","first-page":"674","volume":"34","author":"Ritter K.","year":"2015","journal-title":"J. Shellfish Res."},{"key":"refg39\/ref39","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990110111"},{"key":"refg40\/ref40","doi-asserted-by":"publisher","DOI":"10.3354\/dao065187"},{"key":"refg41\/ref41","doi-asserted-by":"publisher","DOI":"10.1080\/00087114.2005.10589453"},{"key":"refg43\/ref43","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.039537"},{"key":"refg45\/ref45","doi-asserted-by":"publisher","DOI":"10.1007\/BF00397681"},{"key":"refg46\/ref46","doi-asserted-by":"publisher","DOI":"10.1007\/BF00058813"},{"key":"refg47\/ref47","doi-asserted-by":"publisher","DOI":"10.1139\/g92-007"},{"key":"refg48\/ref48","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(98)00457-8"},{"key":"refg49\/ref49","doi-asserted-by":"publisher","DOI":"10.1139\/g94-103"},{"key":"refg50\/ref50","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.0800270"},{"key":"refg52\/ref52","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2109.2010.02541.x"},{"key":"refg53\/ref53","doi-asserted-by":"publisher","DOI":"10.1007\/s11802-014-2318-x"},{"key":"refg54\/ref54","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300033991"}],"container-title":["Genome"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/gen-2017-0173","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/gen-2017-0173","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T14:38:08Z","timestamp":1751467088000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/gen-2017-0173"}},"subtitle":[],"editor":[{"given":"M.","family":"Puertas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1139\/gen-2017-0173"],"URL":"https:\/\/doi.org\/10.1139\/gen-2017-0173","relation":{},"ISSN":["0831-2796","1480-3321"],"issn-type":[{"value":"0831-2796","type":"print"},{"value":"1480-3321","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2]]},"assertion":[{"value":"2017-08-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}