{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T23:55:49Z","timestamp":1784159749548,"version":"3.55.0"},"reference-count":86,"publisher":"Canadian Science Publishing","issue":"6","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Genome"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:p> We aim to overcome the unclear origin of the loquat and elucidate the heterosis mechanism of the triploid loquat. Here we investigated the genetic and epigenetic variations between the triploid plant and its parental lines using amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified fragment length polymorphism (MSAP) analyses. We show that in addition to genetic variations, extensive DNA methylation variation occurred during the formation process of triploid loquat, with the triploid hybrid having increased DNA methylation compared to the parents. Furthermore, a correlation existed between genetic variation and DNA methylation remodeling, suggesting that genome instability may lead to DNA methylation variation or vice versa. Sequence analysis of the MSAP bands revealed that over 53% of them overlap with protein-coding genes, which may indicate a functional role of the differential DNA methylation in gene regulation and hence heterosis phenotypes. Consistent with this, the genetic and epigenetic alterations were associated closely to the heterosis phenotypes of triploid loquat, and this association varied for different traits. Our results suggested that the formation of triploid is accompanied by extensive genetic and DNA methylation variation, and these changes contribute to the heterosis phenotypes of the triploid loquats from the two cross lines. <\/jats:p>","DOI":"10.1139\/gen-2017-0232","type":"journal-article","created":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T12:27:56Z","timestamp":1524572876000},"page":"437-447","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Extensive genetic and DNA methylation variation contribute to heterosis in triploid loquat hybrids"],"prefix":"10.1139","volume":"61","author":[{"given":"Chao","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Horticulture Science for Southern Mountainous Region, Ministry of Education\/College of Horticulture and Landscape Architecture, Southwest University, Tiansheng Road 2, 400715, Chongqing, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingbo","family":"Wang","sequence":"additional","affiliation":[{"name":"CSIRO Agriculture and Food, Clunies Ross Street, Canberra ACT 2061, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingli","family":"Wang","sequence":"additional","affiliation":[{"name":"Technical Advice Station of Economic Crop, Yubei district, Chongqing, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qigao","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Horticulture Science for Southern Mountainous Region, Ministry of Education\/College of Horticulture and Landscape Architecture, Southwest University, Tiansheng Road 2, 400715, Chongqing, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guolu","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Horticulture Science for Southern Mountainous Region, Ministry of Education\/College of Horticulture and Landscape Architecture, Southwest University, Tiansheng Road 2, 400715, Chongqing, P.R. 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