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Given its clinical importance, several major studies have investigated the genomic adaptation of P. aeruginosa in lungs and its transition as acute infections become chronic. However, our knowledge about the diversity and adaptation of the P. aeruginosa genome to non-clinical environments is still fragmentary, in part due to the lack of accurate reference genomes of strains from the numerous environments colonized by the bacterium. Here, we used PacBio long-read technology to sequence the genome of PPF-1, a strain of P. aeruginosa isolated from a dental unit waterline. Generating this closed genome was an opportunity to investigate genomic features that are difficult to accurately study in a draft genome (contigs state). It was possible to shed light on putative genomic islands, some shared with other reference genomes, new prophages, and the complete content of insertion sequences. In addition, four different group II introns were also found, including two characterized here and not listed in the specialized group II intron database. <\/jats:p>","DOI":"10.1139\/gen-2017-0239","type":"journal-article","created":{"date-parts":[[2018,3,16]],"date-time":"2018-03-16T15:47:26Z","timestamp":1521215246000},"page":"359-365","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Unexpected diversity in the mobilome of a <i>Pseudomonas aeruginosa<\/i> strain isolated from a dental unit waterline revealed by SMRT Sequencing"],"prefix":"10.1139","volume":"61","author":[{"given":"Antony T.","family":"Vincent","sequence":"first","affiliation":[{"name":"Institut de biologie int\u00e9grative et des syst\u00e8mes (IBIS), Universit\u00e9 Laval, Quebec City, QC, Canada."},{"name":"Centre de recherche de l\u2019Institut universitaire de cardiologie et de pneumologie de Qu\u00e9bec (CRIUCPQ), Quebec City, QC, Canada."},{"name":"D\u00e9partement de biochimie, de microbiologie et de bio-informatique, Universit\u00e9 Laval, Quebec City, QC, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve J.","family":"Charette","sequence":"additional","affiliation":[{"name":"Institut de biologie int\u00e9grative et des syst\u00e8mes (IBIS), Universit\u00e9 Laval, Quebec City, QC, Canada."},{"name":"Centre de recherche de l\u2019Institut universitaire de cardiologie et de pneumologie de Qu\u00e9bec (CRIUCPQ), Quebec City, QC, Canada."},{"name":"D\u00e9partement de biochimie, de microbiologie et de bio-informatique, Universit\u00e9 Laval, Quebec City, QC, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Barbeau","sequence":"additional","affiliation":[{"name":"D\u00e9partement de stomatologie, Facult\u00e9 de m\u00e9decine dentaire, Universit\u00e9 de Montr\u00e9al, Montreal, QC, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01279-15"},{"key":"refg2\/ref2","doi-asserted-by":"crossref","unstructured":"Al-Nayyef, H., Guyeux, C., Petitjean, M., Hocquet, D., and Bahi, J.M. 2015. 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