{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T00:46:46Z","timestamp":1755218806436,"version":"3.43.0"},"reference-count":52,"publisher":"Canadian Science Publishing","issue":"12","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Genome"],"published-print":{"date-parts":[[2022,12,1]]},"abstract":"<jats:p> The purpose of this study was to study the genetic mechanism of low hydrocyanic acid (HCN) content. The segregation of HCN content trait in fresh stems and leaves was determined in the sorghum ( Sorghum bicolor (L.) Moench)\u2013sudangrass ( Sorghum sudanense (Piper) Stapf) hybrid F<jats:sub>2<\/jats:sub> population ( N\u00a0=\u00a01200), also used to detect a quantitative trait locus (QTL) for HCN content. Our hypothesis was that the additive effect of QTL was negative, showing that QTL was associated with low HCN. In the present research, a total of 11 simple sequence repeats (SSR) polymorphic primers were screened, and four SSR markers associated with low HCN content were developed based on the bulked segregant analysis method. A high-resolution genetic linkage group of the previously known qPA7-1 locus of the low HCN trait was constructed by analyzing different populations, families, and recombinants. Then, the QTL qPA7-1 of sorghum\u2013sudangrass hybrid was fine-mapped to a 203.6\u00a0kb region between markers SORBI4G4-120 and SORBI4G4-680, and seven candidate genes for low HCN were predicted in this region based on sequence comparison with the sorghum reference genome. According to gene annotation, the candidate genes related to low HCN content may be different from those involved in the known regulation mode of sorghum dhurrin biosynthesis and metabolism. <\/jats:p>","DOI":"10.1139\/gen-2021-0114","type":"journal-article","created":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T17:35:47Z","timestamp":1663263347000},"page":"605-619","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Fine mapping of a major QTL <i>qPA7-1<\/i> for low hydrocyanic acid content in sorghum\u2013sudangrass hybrid"],"prefix":"10.1139","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5538-9613","authenticated-orcid":false,"given":"Guofang","family":"Wu","sequence":"first","affiliation":[{"name":"Agricultural College, Inner Mongolia Agricultural University","place":["Hohhot, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxia","family":"Yu","sequence":"additional","affiliation":[{"name":"Agricultural College, Inner Mongolia Agricultural University","place":["Hohhot, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuo","family":"Yu","sequence":"additional","affiliation":[{"name":"Agricultural College, Inner Mongolia Agricultural University","place":["Hohhot, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianqian","family":"Lu","sequence":"additional","affiliation":[{"name":"Agricultural College, Inner Mongolia Agricultural University","place":["Hohhot, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongsheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Agricultural College, Inner Mongolia Agricultural University","place":["Hohhot, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Shi","sequence":"additional","affiliation":[{"name":"Agricultural College, Inner Mongolia Agricultural University","place":["Hohhot, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaqi","family":"Li","sequence":"additional","affiliation":[{"name":"Agricultural College, Inner Mongolia Agricultural University","place":["Hohhot, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingwei","family":"Li","sequence":"additional","affiliation":[{"name":"Agricultural College, Inner Mongolia Agricultural University","place":["Hohhot, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1614\/0043-1745(2001)049[0491:CIWCCA]2.0.CO;2"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1139\/gen-43-6-988"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcv153"},{"key":"refg4\/ref4","first-page":"23","volume-title":"Cyanide: occurrence, characteristics and applications","author":"C\u00e2mara A.C.L.","year":"2013"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302077200"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1206"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.13207\/j.cnki.jnwafu.2015.04.031"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.2307\/4044056"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-050213-040027"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016298100201"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-015-2549-2"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci2015.06.0379"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178177"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90338-3"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1007\/s11032-010-9477-2"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.20546\/ijcmas.2019.809.152"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-020-03560-w"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-697X.2008.00109.x"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036406"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.2134\/agronj1970.00021962006200030025x"},{"key":"refg22\/ref22","first-page":"389","volume":"18","author":"Lu J.","year":"2009","journal-title":"Acta Laser. 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