{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:28:27Z","timestamp":1787167707188,"version":"build-2736575974"},"reference-count":30,"publisher":"Canadian Science Publishing","issue":"11","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"crossref","award":["BT\/PR12987\/INF\/22\/205\/2015"],"award-info":[{"award-number":["BT\/PR12987\/INF\/22\/205\/2015"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Genome"],"published-print":{"date-parts":[[2024,11,1]]},"abstract":"<jats:p>\u03b2-Caryophyllene possesses potential anticancer properties against various cancers, including breast, colon, and lung cancer. Therefore, the essential oil of Ayapana triplinervis, which is rich in \u03b2-caryophyllene, can be a potential herbal remedy for treating cancer. However, molecular and genomic studies on A. triplinervis are still sparse . In this study, we obtained 14.7 Gb of RNA-Seq data from A. triplinervis leaf RNA and assembled 137\u2009554 transcripts with an N50 value of 1437\u00a0bp. We annotated 72\u2009436 (52.7%) transcripts and mapped 10\u2009640 transcripts to 156 biochemical pathways. Among them, 218 were related to terpenoid backbone biosynthesis, while 27 were linked to sesquiterpenoid and triterpenoid pathways. Ninety-four transcripts were annotated in the \u03b2-caryophyllene and lupeol pathways. From these transcripts, for the first time, we identified 25 full-length genes encoding all the 17 enzymes involved in \u03b2-caryophyllene biosynthesis and an additional five genes involved in lupeol biosynthesis. These genes will be useful for the metabolic engineering of \u03b2-caryophyllene and lupeol biosynthesis, not just in A. triplinervis but also in other species.<\/jats:p>","DOI":"10.1139\/gen-2024-0005","type":"journal-article","created":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T17:34:45Z","timestamp":1720805685000},"page":"440-444","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification of full-length genes involved in the biosynthesis of \u03b2-caryophyllene and lupeol from the leaf transcriptome of\n                    <i>Ayapana triplinervis<\/i>"],"prefix":"10.1139","volume":"67","author":[{"family":"Tanuja","sequence":"first","affiliation":[{"name":"College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology","place":["Chennai, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2265-1715","authenticated-orcid":false,"given":"Madasamy","family":"Parani","sequence":"additional","affiliation":[{"name":"College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology","place":["Chennai, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.jams.2012.01.003","volume":"5","author":"Arung T.E.","year":"2012","journal-title":"J. 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