{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T22:45:13Z","timestamp":1788389113757,"version":"build-2803163510"},"reference-count":29,"publisher":"Canadian Science Publishing","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/deed.en_GB"}],"funder":[{"name":"Centre de recherche en infectiologie porcine et aviaire"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Genome"],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:p> Staphylococcus hyicus is the etiological agent of exudative epidermitis (EE) in suckling and weaned piglets, which is an endemic disease of concern for animal health and welfare on swine farms. Staphylococcus hyicus is not widely studied, and there is no recent scientific literature on Canadian strains. In a preliminary study, we presented an Eastern Canadian S.\u00a0hyicus strain isolated from an EE case and bearing a plasmidic tet(L) gene conferring resistance to tetracyclines, one of the most commonly used antibiotics in Canadian swine farms. The current study characterized the tet(L)-bearing plasmids present in S. hyicus isolates from pigs in Eastern Canadian to better understand the potential vectors for this gene dissemination. Eleven S.\u00a0hyicus isolates harboring tet(L) as the only tetracycline resistance gene were sequenced using Oxford Nanopore Technology and analyzed. Eight different tet(L)-bearing plasmids were identified that share similarities with Staphylococcus aureus sequences available in the National Center for Biotechnology Information. Only one of the plasmids was present in more than one isolate and two isolates carried tet(L) on their chromosome. This suggests a high diversity of tet(L)-bearing plasmids in S.\u00a0hyicus and the possible need to analyze swine farm samples over time to understand the dynamics of tet(L) in S.\u00a0hyicus. <\/jats:p>","DOI":"10.1139\/gen-2025-0007","type":"journal-article","created":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T21:34:08Z","timestamp":1757108048000},"page":"1-7","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Diversity of <i>tet<\/i>(L)-bearing plasmids found in Eastern Canadian <i>Staphylococcus hyicus<\/i> isolates from swine"],"prefix":"10.1139","volume":"68","author":[{"given":"Cynthia","family":"Gagn\u00e9-Thivierge","sequence":"first","affiliation":[{"name":"Universit\u00e9 Laval","place":["Quebec City, Canada"]},{"name":"Universit\u00e9 Laval","place":["Quebec City, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Lou","family":"Gauthier","sequence":"additional","affiliation":[{"name":"Laboratoire de sant\u00e9 animale, Minist\u00e8re de l'agriculture, des p\u00eacheries et de l'alimentation du Qu\u00e9bec (MAPAQ)","place":["Saint-Hyacinthe, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Denicourt","sequence":"additional","affiliation":[{"name":"Centre de recherche en infectiologie porcine et avicole (CRIPA) - Fonds de recherche du Qu\u00e9bec \u2013 secteur Nature et technologies","place":["Saint-Hyacinthe, Canada"]},{"name":"Universit\u00e9 de Montr\u00e9al","place":["Saint-Hyacinthe, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-\u00c8ve","family":"Lambert","sequence":"additional","affiliation":[{"name":"Centre de recherche en infectiologie porcine et avicole (CRIPA) - Fonds de recherche du Qu\u00e9bec \u2013 secteur Nature et technologies","place":["Saint-Hyacinthe, Canada"]},{"name":"Universit\u00e9 de Montr\u00e9al","place":["Saint-Hyacinthe, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1338-5785","authenticated-orcid":false,"given":"Antony T.","family":"Vincent","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Laval","place":["Quebec City, Canada"]},{"name":"Centre de recherche en infectiologie porcine et avicole (CRIPA) - Fonds de recherche du Qu\u00e9bec \u2013 secteur Nature et technologies","place":["Saint-Hyacinthe, Canada"]},{"name":"Universit\u00e9 Laval","place":["Quebec City, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0199-2852","authenticated-orcid":false,"given":"Steve J.","family":"Charette","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Laval","place":["Quebec City, Canada"]},{"name":"Universit\u00e9 Laval","place":["Quebec City, Canada"]},{"name":"Centre de recherche en infectiologie porcine et avicole (CRIPA) - Fonds de recherche du Qu\u00e9bec \u2013 secteur Nature et technologies","place":["Saint-Hyacinthe, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2021.1878688"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241914587"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.2.232-260.2001"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1111\/J.1365-3164.2012.01093.X"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1002\/9781119350927.CH60"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.00626-23"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts503"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00811-19"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0072-8"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw152"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btab705"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.1122679"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.3390\/ANTIBIOTICS12010078"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.4315\/JFP-21-431"},{"issue":"2","key":"refg18\/ref18","first-page":"139","volume":"54","author":"Park J.","year":"2013","journal-title":"Can. Vet. J."},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00088-17"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","unstructured":"Public Health Agency of Canada. 2022. Canadian Antimicrobial Resistance Surveillance system (CARSS): report. 2022. doi:10.58333\/e241022.","DOI":"10.58333\/e241022"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.02.034"},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(90)90058-4"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2011.06275.x"},{"key":"refg24\/ref24","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.ARBA-0010-2017"},{"key":"refg25\/ref25","doi-asserted-by":"publisher","DOI":"10.1099\/MGEN.0.000685"},{"key":"refg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr039"},{"key":"refg27\/ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2024.110083"},{"key":"refg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.01310-21"},{"key":"refg29\/ref29","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1094067"},{"key":"refg30\/ref30","doi-asserted-by":"publisher","DOI":"10.1002\/ADVS.202103388"}],"container-title":["Genome"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/gen-2025-0007","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/gen-2025-0007","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T13:33:35Z","timestamp":1759325615000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/gen-2025-0007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,1]]},"references-count":29,"alternative-id":["10.1139\/gen-2025-0007"],"URL":"https:\/\/doi.org\/10.1139\/gen-2025-0007","relation":{},"ISSN":["0831-2796","1480-3321"],"issn-type":[{"value":"0831-2796","type":"print"},{"value":"1480-3321","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,1]]},"assertion":[{"value":"2025-09-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}