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Merr.) cultivation into Western Canada is constrained by distinct abiotic stressors, including shorter growing seasons and drought. Heat shock proteins (HSPs) are critical regulators of stress adaptation; however, characterizing their expression in field conditions is often confounded by significant annual environmental variability. In this study, we integrated differential expression analysis, consensus Weighted Gene Co-expression Network Analysis (WGCNA), and rigorous protein\u2013protein interactomic modeling across 4 years of field data (2018\u20132021) involving 10 genotypes grown in Eastern and Western Canada. By filtering temporal noise, we identified a stable, region-specific module that is computationally predicted to resolve into a highly coordinated physical chaperone complex. Subcellular characterization of this network revealed a robust core of chaperones targeting mitochondria and chloroplasts. Crucially, high-confidence predictions identified a physical interaction between a mitochondrial HSP60 hub and a nuclear domains rearranged methyltransferase, suggesting this chaperone complex potentially bridges localized organelle defense with systemic epigenetic regulation to safeguard metabolic capacity. 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