{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T22:34:06Z","timestamp":1782513246832,"version":"3.54.5"},"reference-count":35,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T00:00:00Z","timestamp":1306886400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Appl. Physiol. Nutr. Metab."],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:p>Our aim was to investigate whether trans-resveratrol (t-resveratrol), a red wine constituent known for its cardioprotective effects, was able to influence CD40 ligand (CD40L) and its receptor CD40 in platelets of hypercholesterolemic rats. Sixty Wistar rats were divided into 5 groups: control (C), ethanol (E), t-resveratrol (R), hypercholesterolemia (HC), and hypercholesterolemia plus t-resveratrol (HCR). Rats in the C, E, and R groups were fed a normal diet for 80\u00a0days. For 20\u00a0days before sacrifice, we intraperitoneally (i.p.) administered 0.1\u00a0mL ethanol (50% v\/v) to the E group, and 0.1\u00a0mL t-resveratrol (20\u00a0mg\u00b7kg<jats:sup>\u20131<\/jats:sup>\u00b7day<jats:sup>\u20131<\/jats:sup>) to the R group. Rats in the HC and HCR groups were fed a 5% cholesterol diet for 80\u00a0days. Rats in the HCR group were administered i.p.\u00a00.1\u00a0mL t-resveratrol (20\u00a0mg\u00b7kg<jats:sup>\u20131<\/jats:sup>\u00b7day<jats:sup>\u20131<\/jats:sup>) for 20\u00a0days before sacrifice. Serum levels of total cholesterol (TC), low-density lipoprotein (LDL-C), high-density lipoprotein (HDL-C), very low-density lipoprotein (VLDL-C), and total triglycerides (TG) were assayed with a commercial colorimetric kit. Platelet P-selectin, CD40, and CD40L expression was determined by flow cytometry. sCD40L and IL6 levels were measured by ELISA. In the HC group, we observed a significant increase in serum TC, LDL-C, VLDL-C, TG, sCD40, and IL-6 levels and platelet activation markers compared with levels in the control group. However, t-resveratrol administration to the HC group (HCR group) attenuated the increase in lipids, sCD40, and IL-6 and down-regulated platelet P-selectin, CD40, and CD40L expressions. A positive correlation was found for serum lipids and all the platelet activation markers. Our study showed that the CD40\u2013CD40L dyad is up-regulated in the presence of hypercholesterolemia and that t-resveratrol administration down-regulated the increase.<\/jats:p>","DOI":"10.1139\/h11-022","type":"journal-article","created":{"date-parts":[[2011,5,20]],"date-time":"2011-05-20T21:56:10Z","timestamp":1305928570000},"page":"323-330","source":"Crossref","is-referenced-by-count":40,"title":["Effect of resveratrol on platelet activation in hypercholesterolemic rats: CD40\u2013CD40L system as a potential target"],"prefix":"10.1139","volume":"36","author":[{"given":"Ay\u015fe Ye\u015fim","family":"G\u00f6\u00e7men","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Bozok University, 66200 Yozgat, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Durmus","family":"Burgucu","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saadet","family":"G\u00fcm\u00fc\u015fl\u00fc","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1021\/jf048177q"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.12.140"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1024\/0300-9831.78.1.3"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2008.08.017"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1159\/000093226"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(99)00410-5"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1161\/hc4501.099312"},{"issue":"1","key":"refg8\/ref8","first-page":"19","volume":"13","author":"Haugen E.","year":"2008","journal-title":"Exp. Clin. Cardiol"},{"issue":"4","key":"refg9\/ref9","first-page":"288","volume":"24","author":"H\u00e9zard N.","year":"1999","journal-title":"J. Mal. Vasc."},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2004.04.037"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000070111.98158.6C"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2009.03.026"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2007.02412.x"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2004.08.016"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-05-2005"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000129773.70647.94"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvp139"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1001\/jama.286.17.2107"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(03)00469-7"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9149(99)00489-0"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-5688(09)71816-1"},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-3848(01)00279-1"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2006.10.018"},{"key":"refg24\/ref24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.13.6930"},{"key":"refg25\/ref25","doi-asserted-by":"publisher","DOI":"10.1021\/jf0580364"},{"key":"refg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2008.09.006"},{"issue":"3","key":"refg27\/ref27","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1111\/j.1365-2141.2007.06788.x","volume":"139","author":"Shen M.Y.","year":"2007","journal-title":"Br. J. Haematol."},{"key":"refg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00962.2008"},{"key":"refg29\/ref29","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1992.32692367196.x"},{"key":"refg30\/ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2010.08.001"},{"key":"refg31\/ref31","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.08.040190.001345"},{"issue":"1","key":"refg32\/ref32","first-page":"77","volume":"9","author":"Wang Z.","year":"2002","journal-title":"Int. J. Mol. Med."},{"issue":"3","key":"refg33\/ref33","first-page":"378","volume":"115","author":"Wang Z.","year":"2002","journal-title":"Chin. Med. J. (Engl.)"},{"issue":"7","key":"refg34\/ref34","first-page":"1101","volume":"117","author":"Yan J.C.","year":"2004","journal-title":"Chin. Med. J. (Engl.)"},{"key":"refg35\/ref35","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2008.01.009"}],"container-title":["Applied Physiology, Nutrition, and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/full-xml\/10.1139\/h11-022","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/h11-022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,6]],"date-time":"2024-04-06T22:52:54Z","timestamp":1712443974000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/h11-022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,6]]}},"alternative-id":["10.1139\/h11-022"],"URL":"https:\/\/doi.org\/10.1139\/h11-022","relation":{},"ISSN":["1715-5312","1715-5320"],"issn-type":[{"value":"1715-5312","type":"print"},{"value":"1715-5320","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,6]]}}}