{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T05:03:24Z","timestamp":1772773404462,"version":"3.50.1"},"reference-count":41,"publisher":"Canadian Science Publishing","issue":"6","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Appl. Physiol. Nutr. Metab."],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p> The deleterious bone effects of mechanical unloading have been suggested to be due to oxidative stress and (or) inflammation. Resveratrol has both antioxidant and anti-inflammatory properties; therefore, the study's objective was to determine whether providing resveratrol in the low supplementation range for a short duration prevents bone loss during mechanical unloading. Mature (6 months old) Fischer 344 \u00d7 Brown Norway male rats were hindlimb-suspended (HLS) or kept ambulatory for 14 days. Rats were provided either trans-resveratrol (RES; 12.5 mg\/kg body mass per day) or deionized distilled water by oral gavage for 21 days (7 days prior to and during the 14 days of HLS). Bone mass was measured by dual energy X-ray absorptiometry. Bone microstructure was determined by microcomputed tomography. HLS of rats resulted in femur trabecular bone deterioration. Resveratrol supplementation did not attenuate trabecular bone deterioration in HLS rats. Unexpectedly, HLS\u2013RES rats had the lowest tibial bone mineral content (P &lt; 0.05), calcium content and lower cortical thickness (P &lt; 0.05), and increased porosity compared with HLS\/control rats. Plasma osteocalcin was also lower (P &lt; 0.04) in HLS\/resveratrol rats. There were no significant effects on plasma C-reactive protein, a marker of systemic inflammation, or total antioxidant capacity. However, HLS\u2013RES rats showed a negative relationship (r<jats:sup>2<\/jats:sup> = 0.69, P = 0.02) between plasma osteocalcin and thiobarbituric acid reactive substances, a marker of lipid peroxidation. Based on the results, resveratrol supplementation of 6-month-old HLS male rats had no bone protective effects and possibly even detrimental bone effects. <\/jats:p>","DOI":"10.1139\/h2012-099","type":"journal-article","created":{"date-parts":[[2012,11,23]],"date-time":"2012-11-23T15:11:38Z","timestamp":1353683498000},"page":"1179-1188","source":"Crossref","is-referenced-by-count":24,"title":["Resveratrol supplementation influences bone properties in the tibia of hindlimb-suspended mature Fisher 344 \u00d7 Brown Norway male rats"],"prefix":"10.1139","volume":"37","author":[{"given":"Stephanie M.","family":"Durbin","sequence":"first","affiliation":[{"name":"Human Nutrition and Foods, Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26505, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janna R.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Laboratory of Muscle Biology and Sacropenia, Division of Exercise Physiology, West Virginia University, Morgantown, WV 26505, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Laboratory of Muscle Biology and Sacropenia, Division of Exercise Physiology, West Virginia University, Morgantown, WV 26505, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph C.","family":"Gigliotti","sequence":"additional","affiliation":[{"name":"Human Nutrition and Foods, Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26505, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen E.","family":"Alway","sequence":"additional","affiliation":[{"name":"Laboratory of Muscle Biology and Sacropenia, Division of Exercise Physiology, West Virginia University, Morgantown, WV 26505, USA."},{"name":"Center for Cardiovascular and Respiratory Sciences, West Virginia University, Morgantown, WV 26505, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet C.","family":"Tou","sequence":"additional","affiliation":[{"name":"Human Nutrition and Foods, Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26505, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200500022"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0351156"},{"key":"refg3\/ref3","unstructured":"Armstrong, D., and Browne, R. 1994. The analysis of free radicals, lipid peroxidases, antioxidant enzymes and compounds related to oxidative stress as applied to the clinical chemistry laboratory. In Free radicals in diagnostic medicine: a systems approach to laboratory, technology, clinical correlations, and antioxidant therapy. Edited by D. Armstrong. Plenum Press, New York. pp. 43\u201358."},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-010-0013-4"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(02)00785-8"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1021\/jf0112973"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.2011.03966.x"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.2165\/00002512-199813060-00002"},{"issue":"1","key":"refg11\/ref11","first-page":"49","volume":"24","author":"Fotiou S.","year":"2010","journal-title":"In Vivo"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114485"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.2165\/00007256-200232070-00005"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1007\/s00774-010-0187-y"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20650"},{"key":"refg16\/ref16","unstructured":"Institute of Laboratory Animal Resources Commission on Life Sciences. 1996. Guide for the care and use of laboratory animals. National Academy Press, Washington, DC. pp. 21\u201355."},{"issue":"3","key":"refg17\/ref17","first-page":"155","volume":"9","author":"Ip T.Y.","year":"2009","journal-title":"J. Musculoskelet. Neuronal Interact."},{"issue":"11","key":"refg18\/ref18","first-page":"1233","volume":"40","author":"Irshad M.","year":"2002","journal-title":"Indian J. Exp. Biol."},{"issue":"2","key":"refg19\/ref19","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1093\/jn\/132.2.257","volume":"132","author":"Juan M.E.","year":"2002","journal-title":"J. Nutr."},{"issue":"4","key":"refg20\/ref20","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1093\/jn\/135.4.757","volume":"135","author":"Juan M.E.","year":"2005","journal-title":"J. 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