{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T05:58:22Z","timestamp":1762408702581},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"1","license":[{"start":{"date-parts":[[1971,1,1]],"date-time":"1971-01-01T00:00:00Z","timestamp":31536000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1971,1,1]]},"abstract":"<jats:p> Using cell-free extracts of Nitrobacter agilis, we investigated the effects of CIPC and eptam on the oxidative phosphorylation coupled to nitrite oxidation as well as upon the reduced nicotinamide adenine dinucleotide (NADH<jats:sub>2<\/jats:sub>) oxidation to determine possible effects of the herbicides upon electron transport. Both CIPC and eptam exerted an uncoupling effect on the oxidative phosphorylation linked to nitrite oxidation. Although nitrite oxidation was inhibited, an intensely severe inhibition of phosphorylation caused a 50% reduction in the P\/O ratio (\u03bcmoles of <jats:sup>32<\/jats:sup>P esterified per \u03bcatom of oxygen consumed) at about 2.3\u2002\u00d7\u200210<jats:sup>\u22124<\/jats:sup>\u2002M CIPC and at 8\u2002\u00d7\u200210<jats:sup>\u22124<\/jats:sup>\u2002M eptam. Likewise, 2,4-dinitrophenol (DNP), a classical phosphorylation uncoupler, affected the oxidative phosphorylation in N. agilis. Although oxygen uptake was inhibited, a 45% reduction in the P\/O ratio occurred at 2.5\u2002\u00d7\u200210<jats:sup>\u22124<\/jats:sup>\u2002M DNP because of a greater effect on phosphate esterification. Neither CIPC nor eptam affected reduced nicotinamide adenine dinucleotide (NADH<jats:sub>2<\/jats:sub>) oxidase activity. <\/jats:p>","DOI":"10.1139\/m71-008","type":"journal-article","created":{"date-parts":[[2010,2,8]],"date-time":"2010-02-08T16:48:17Z","timestamp":1265647697000},"page":"47-51","source":"Crossref","is-referenced-by-count":5,"title":["The effect of two herbicides (CIPC and eptam) on oxidative phosphorylation by <i>Nitrobacter agilis<\/i>"],"prefix":"10.1139","volume":"17","author":[{"given":"C. L.","family":"Winely","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. L. San","family":"Clemente","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m71-008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T05:05:48Z","timestamp":1578287148000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m71-008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1971,1,1]]}},"alternative-id":["10.1139\/m71-008"],"URL":"https:\/\/doi.org\/10.1139\/m71-008","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,1,1]]}}}