{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T11:03:28Z","timestamp":1760267008670},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[1971,8,1]],"date-time":"1971-08-01T00:00:00Z","timestamp":49852800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1971,8,1]]},"abstract":"<jats:p> This investigation was prompted by the reported formation of HCN from isoleucine by way of the cyanogenic glycoside lotaustralin in a psychrophilic basidiomycete. In comparable experiments we found steam distillates from cultures incubated with L-isoleucine-U-<jats:sup>14<\/jats:sup>C were radioactive but this was shown not to be due to H<jats:sup>14<\/jats:sup>CN, by removal of the HCN as AgCN. This was confirmed using isoleucine-2-<jats:sup>14<\/jats:sup>C from which H<jats:sup>14<\/jats:sup>CN recovery was negligible. Evidence was obtained that the steam volatile radioactive material regarded by other workers as H<jats:sup>14<\/jats:sup>CN consisted largely of organic acids. Hence isoleucine cannot be considered to be a precursor of HCN in this fungus and the occurrence of lotaustralin as an intermediate is therefore doubtful. Under the same conditions HCN was shown to arise from carbon-2 of glycine, confirming previous reports from this laboratory. <\/jats:p>","DOI":"10.1139\/m71-168","type":"journal-article","created":{"date-parts":[[2010,2,8]],"date-time":"2010-02-08T16:48:56Z","timestamp":1265647736000},"page":"1061-1066","source":"Crossref","is-referenced-by-count":9,"title":["The amino acid source of HCN in cultures of a psychrophilic basidiomycete"],"prefix":"10.1139","volume":"17","author":[{"given":"E. W. B.","family":"Ward","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. D.","family":"Thorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. N.","family":"Starratt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m71-168","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T05:06:22Z","timestamp":1578287182000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m71-168"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,8,1]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1971,8,1]]}},"alternative-id":["10.1139\/m71-168"],"URL":"https:\/\/doi.org\/10.1139\/m71-168","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,8,1]]}}}