{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:13:52Z","timestamp":1762917232390},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[1972,5,1]],"date-time":"1972-05-01T00:00:00Z","timestamp":73526400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1972,5,1]]},"abstract":"<jats:p> Hamsters injected subcutaneously with a single \"low-dose\" inoculum (10 mouse LD<jats:sub>50<\/jats:sub>) of Powassan virus developed viremia titering 10<jats:sup>6.2<\/jats:sup> mouse LD<jats:sub>50<\/jats:sub> per milliliter of blood whereas hamsters receiving a \"high-dose\" inoculum of 10<jats:sup>5.0<\/jats:sup> mouse LD<jats:sub>50<\/jats:sub> of virus developed a viremia of only 10<jats:sup>4.5<\/jats:sup> mouse LD<jats:sub>50<\/jats:sub> per milliliter.Hemagglutination-inhibiting (HI) antibodies were first detected 7 days following the \"low-dose\" inoculation and attained maximum titers of 160. The HI antibody response to the \"high-dose\" virus inoculation began on the 5th day, at a time when viremia was present, and antibody titers did not exceed 40. Sucrose-density analysis and 2-mercaptoethanol treatment of sera revealed that IgM antibodies were induced by both inocula but animals receiving a low dose of virus produced higher IgG responses than did animals receiving a high dose of virus. Neutralizing antibodies, which did not appear until 30 days post inoculation, were present at a log neutralizing index (NI) of 2.0 at 60 days in animals receiving 10 mouse LD<jats:sub>50<\/jats:sub> of virus and at a log NI of 1.0 in those receiving 10<jats:sup>5.0<\/jats:sup> mouse LD<jats:sub>50<\/jats:sub>. The NI of IgG was equal to the index for total gamma globulin in samples that contained both IgM and IgG as demonstrated by the HI test.Animals originally exposed to a \"low-dose\" inoculum produced a more prolonged anamnestic response when challenged 63 days later with 10<jats:sup>5.0<\/jats:sup> mouse LD<jats:sub>50<\/jats:sub> of virus than did animals receiving a second inoculum containing only 10<jats:sup>3.0<\/jats:sup> or 10 mouse LD<jats:sub>50<\/jats:sub> of virus. In contrast, poor secondary antibody responses were elicited by challenge doses of 10<jats:sup>5.0<\/jats:sup>, 10<jats:sup>3.0<\/jats:sup>, or 10 mouse LD<jats:sub>50<\/jats:sub> of virus in animals that originally had received a \"high-dose\" of virus. IgM and IgG classes of antibody were induced in all secondary responses and the log NI of each group of animals was elevated by about 1 during anamnesis. <\/jats:p>","DOI":"10.1139\/m72-103","type":"journal-article","created":{"date-parts":[[2010,2,8]],"date-time":"2010-02-08T16:49:55Z","timestamp":1265647795000},"page":"655-661","source":"Crossref","is-referenced-by-count":6,"title":["Dose-dependent viremia and the differential immunoglobulin response of hamsters to Powassan virus"],"prefix":"10.1139","volume":"18","author":[{"given":"Max A.","family":"Chernesky","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia J.","family":"Whittaker-Haines","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m72-103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T05:05:07Z","timestamp":1578287107000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m72-103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1972,5,1]]}},"alternative-id":["10.1139\/m72-103"],"URL":"https:\/\/doi.org\/10.1139\/m72-103","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,5,1]]}}}