{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T11:40:34Z","timestamp":1648640434593},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[1975,3,1]],"date-time":"1975-03-01T00:00:00Z","timestamp":162864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1975,3,1]]},"abstract":"<jats:p> Chemostat cultures of Erwinia amylovora 595, grown in mineral salts \u2013 nicotinic acid medium at 30 \u00b0C, and limited by D-glucose concentrations in the presence of dissolved oxygen tensions (D.O.T.) greater than about 6\u2002mm Hg, became limited by oxygen availability below about 4\u2002mm Hg. This latter limitation was accompanied by a marked increase in acid production as the D.O.T. was depressed. The transition between D-glucose- and oxygen-limitation was also characterized by a maximum in succinate oxidase activity, and a minimum in the in situ respiration. D-Glyceraldehyde-3-phosphate dehydrogenase and D-fructose-1,6-diphosphate aldolase showed small reductions in specific activity in the region 4\u20136\u2002mm Hg D.O.T., but further reduction to 2\u2002mm Hg resulted in a marked increase in the specific activity of aldolase. Malate dehydrogenase followed the converse trend, and attained very low activity levels when the D.O.T. decreased beyond the lower limits of detection. The in situ respiration was maximal at 2\u2002mm Hg D.O.T., while potential respiration values were minimal at 2\u2002mm Hg, and maximal at about 8\u2002mm Hg D.O.T. The in situ respiration rate was proportional to dilution rate (D), in presence of excess oxygen, up to 0.18\u2002h<jats:sup>\u22121<\/jats:sup>, after which a marked diminution occurred and continued until the wash-out rate was attained. Succinate oxidase activity decreased with increase in dilution rate, but remained constant above D\u2002=\u20020.18\u2002h<jats:sup>\u22121<\/jats:sup>. Malate dehydrogenase showed a persistent decline with increase in dilution rate, while D-glyceraldehyde-3-phosphate activity increased somewhat at higher dilution rates. The data are interpreted in terms of two transition points, at 6 and 2\u2002mm Hg D.O.T., and of a change from respiratory to fermentative metabolism at low D.O.T., and at high dilution rates. <\/jats:p>","DOI":"10.1139\/m75-049","type":"journal-article","created":{"date-parts":[[2010,4,30]],"date-time":"2010-04-30T19:03:22Z","timestamp":1272654202000},"page":"343-352","source":"Crossref","is-referenced-by-count":3,"title":["Variation in the activity levels of selected enzymes of <i>Erwinia amylovora<\/i> 595 in response to changes in dissolved oxygen tension and growth rate of <scp>D<\/scp>-glucose-limited chemostat cultures"],"prefix":"10.1139","volume":"21","author":[{"given":"D. A.","family":"Farago","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. N.","family":"Gibbins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m75-049","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T05:04:05Z","timestamp":1578287045000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m75-049"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1975,3,1]]}},"alternative-id":["10.1139\/m75-049"],"URL":"https:\/\/doi.org\/10.1139\/m75-049","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,3,1]]}}}