{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T21:58:20Z","timestamp":1759010300605},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"10","license":[{"start":{"date-parts":[[1975,10,1]],"date-time":"1975-10-01T00:00:00Z","timestamp":181353600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1975,10,1]]},"abstract":"<jats:p> During bacteriophage studies on Haemophilus influenzae, it was observed that encapsulated type b and unencapsulated Rb strains released a bactericidal substance active against types a, c, d, e, and f H. influenzae, non-typable H. influenzae strains, other Haemophilus species, and certain members of the Enterobacteriaceae. The bactericidal activity was assayed by a plaque test utilizing an Rd strain as an indicator lawn and was also demonstrated in mixed broth cultures of a producer strain and an indicator strain. Immediate lysis of sensitive bacteria by the factor was not evident. The factor is sensitive to trypsin but resistant to deoxyribonuclease, treatment with 2-mercaptoethanol, lipase, \u03b1-amylase, and heating in a 100 \u00b0C water bath for 20\u2002min. The activity is not dependent upon increased Ca<jats:sup>2+<\/jats:sup> or Mg<jats:sup>2+<\/jats:sup> concentration as is necessary for HP1C1 and S2 phage propagation. The bactericidal factor is not pelleted by high-speed centrifugation at 150\u2002000\u2002\u00d7\u2002g for 6\u2002h. Treatment with ultraviolet light or mitomycin C does not result in observable phage, phage-like particles, or increased bactericidal activity. The bactericidal factor is not a typical small molecular weight \"colicin-like\" bacteriocin in that it is not inducible, has a wider range of activity, and does not kill by \"single-hit\" kinetics. On preliminary characterization, it is a thermostable protein toxic to certain bacterial strains. <\/jats:p>","DOI":"10.1139\/m75-232","type":"journal-article","created":{"date-parts":[[2010,4,30]],"date-time":"2010-04-30T15:03:58Z","timestamp":1272639838000},"page":"1587-1594","source":"Crossref","is-referenced-by-count":19,"title":["Bactericidal substance produced by <i>Haemophilus influenzae b<\/i>"],"prefix":"10.1139","volume":"21","author":[{"given":"R. A.","family":"Venezia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. G.","family":"Robertson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m75-232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T00:10:07Z","timestamp":1578269407000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m75-232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,10,1]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1975,10,1]]}},"alternative-id":["10.1139\/m75-232"],"URL":"https:\/\/doi.org\/10.1139\/m75-232","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,10,1]]}}}