{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T17:22:04Z","timestamp":1649179324055},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[1977,3,1]],"date-time":"1977-03-01T00:00:00Z","timestamp":226022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1977,3,1]]},"abstract":"<jats:p> Properties of the glutamate uptake system of Thiobacillus novellus were investigated using intact cells. Two kinetically distinguishable components were found and designated as the high-affinity (HA) component with an apparent K<jats:sub>m<\/jats:sub> of 33\u2002\u03bcM, and the low-affinity (LA) component with a K<jats:sub>m<\/jats:sub> of 18\u2002mM. Glutamate accumulation was temperature-sensitive and could be inhibited by the addition of a wide range of inhibitors including cyanide, dinitrophenol (DNP), iodoacetate, mercuric chloride, sodium azide, carbonyl cyanide m-chlorophenyl hydrazone (CCCP), and dicyclohexylcarbodiimide (DCCD). The complex kinetics of the uptake process may be due to the rapid incorporation of added glutamate into a small peptide. This soluble peptide was formed rapidly after glutamate addition, and accumulated in whole cells. <\/jats:p>","DOI":"10.1139\/m77-040","type":"journal-article","created":{"date-parts":[[2010,4,30]],"date-time":"2010-04-30T19:15:56Z","timestamp":1272654956000},"page":"271-277","source":"Crossref","is-referenced-by-count":1,"title":["Glutamate uptake in <i>Thiobacillus novellus<\/i>"],"prefix":"10.1139","volume":"23","author":[{"given":"Daryl J.","family":"Hoban","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald M.","family":"Lyric","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m77-040","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T05:08:06Z","timestamp":1578287286000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m77-040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1977,3,1]]}},"alternative-id":["10.1139\/m77-040"],"URL":"https:\/\/doi.org\/10.1139\/m77-040","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,3,1]]}}}