{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T23:19:17Z","timestamp":1648768757373},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"12","license":[{"start":{"date-parts":[[1977,12,1]],"date-time":"1977-12-01T00:00:00Z","timestamp":249782400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1977,12,1]]},"abstract":"<jats:p> A sterile glucose\u2013salts broth fortified with various metabolic inhibitors and nutritional supplements was inoculated with conidia of Penicillium rubrum P3290, and incubated quiescently at 28 \u00b0C for 14 days. Potassium sulfite and sodium metabisulfite at all test concentrations caused moderate reduction in rubratoxin formation; at high concentrations (\u22652.7\u2002\u00d7\u200210<jats:sup>\u22122<\/jats:sup>\u2002M) accumulation of fungal tissue was also retarded. Production of rubratoxin and cell mass was inhibited by p-aminobenzoic acid; syntheses of toxin were completely blocked by 7.5\u2002\u00d7\u200210<jats:sup>\u22122<\/jats:sup>\u2002M of the vitamin. Effects of sodium fluoride on P. rubrum cultures grown on inorganic nitrogen sources varied from inhibition of mold growth and (or) rubratoxin A production to reduction in formation of rubratoxin B. With organic nitrogen sources, fluoride caused a 30 and 60% reduction in synthesis of rubratoxins A and B, respectively. Sodium acetate at all test concentrations enhanced formation of rubratoxin; mold growth was enhanced when acetate concentration was \u22656.0\u2002\u00d7\u200210<jats:sup>\u22122<\/jats:sup>\u2002M. A moderate reduction in mold growth was caused by lower acetate concentrations (1.2\u2002\u00d7\u200210<jats:sup>\u22122<\/jats:sup>\u2002M or 2.4\u2002\u00d7\u200210<jats:sup>\u22122<\/jats:sup>\u2002M). Sodium arsenite and iodoacetate at test concentrations blocked mold growth and toxin formation; sodium azide and 2,4-dinitrophenol caused a marked reduction in mold growth but inhibited toxin formation completely. However, sodium azide permitted slight growth and toxin formation when mold cultures were incubated for 28 days. <\/jats:p>","DOI":"10.1139\/m77-244","type":"journal-article","created":{"date-parts":[[2010,4,30]],"date-time":"2010-04-30T19:16:50Z","timestamp":1272655010000},"page":"1695-1699","source":"Crossref","is-referenced-by-count":0,"title":["Bioproduction of rubratoxin in a glucose\u2013mineral salts broth with nutritional supplements and metabolic inhibitors"],"prefix":"10.1139","volume":"23","author":[{"given":"C. Obi","family":"Emeh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elmer H.","family":"Marth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m77-244","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T05:06:58Z","timestamp":1578287218000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m77-244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,12,1]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1977,12,1]]}},"alternative-id":["10.1139\/m77-244"],"URL":"https:\/\/doi.org\/10.1139\/m77-244","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,12,1]]}}}