{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T10:46:49Z","timestamp":1697021209239},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[1982,3,1]],"date-time":"1982-03-01T00:00:00Z","timestamp":383788800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1982,3,1]]},"abstract":"<jats:p> The assimilation of selenite by Escherichia coli involves a transport process specific for this anion. Cystine, a repressor of sulfate and selenate uptake, when added to the growth medium, had no effect on selenite uptake; nor did sulfite inhibit assimilation of selenite. Cells grown aerobically in a basic salts medium transported selenite at an initial rate of 0.14\u2002\u03bcmol\u2219g<jats:sup>\u22121<\/jats:sup>\u2219min<jats:sup>\u22121<\/jats:sup> and a V<jats:sub>max<\/jats:sub> of 393.0\u2002\u03bcmol\u2219g<jats:sup>\u22121<\/jats:sup>\u2219min<jats:sup>\u22121<\/jats:sup>. In contrast, cells grown in a medium that contained the trace elements necessary for synthesis of the selenoenzyme formate dehydrogenase took up selenite at a significantly faster rate (initial rate\u2002=\u20020.27\u2002\u03bcmol\u2219g<jats:sup>\u22121<\/jats:sup>\u2219min<jats:sup>\u22121<\/jats:sup>, V<jats:sub>max<\/jats:sub>\u2002=\u2002658.2\u2002\u03bcmol\u2219g<jats:sup>\u22121<\/jats:sup>\u2219min<jats:sup>\u22121<\/jats:sup>). K<jats:sub>m<\/jats:sub> values for the transport process in the two media, however, were found to be similar. The results suggest the existence of a metabolic pathway, specific for selenite, responsible for the incorporation of selenium into formate dehydrogenase. <\/jats:p>","DOI":"10.1139\/m82-045","type":"journal-article","created":{"date-parts":[[2010,5,20]],"date-time":"2010-05-20T14:28:09Z","timestamp":1274365689000},"page":"307-310","source":"Crossref","is-referenced-by-count":24,"title":["Selective assimilation of selenite by <i>Escherichia coli<\/i>"],"prefix":"10.1139","volume":"28","author":[{"given":"Terence A.","family":"Brown","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Shrift","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m82-045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T00:12:37Z","timestamp":1578269557000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m82-045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1982,3,1]]}},"alternative-id":["10.1139\/m82-045"],"URL":"https:\/\/doi.org\/10.1139\/m82-045","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,3,1]]}}}