{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T22:32:48Z","timestamp":1726439568567},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"6","license":[{"start":{"date-parts":[[1983,6,1]],"date-time":"1983-06-01T00:00:00Z","timestamp":423273600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1983,6,1]]},"abstract":"<jats:p> The efficacy of the acridine orange (AO), gram (G), and methylene blue (MB) microscopic procedures was analyzed using positive blood cultures monitored by a radiometric procedure (Bactec) in children. Sixty-eight positive cultures included the following isolates: Haemophilus influenzae type b, Neisseria meningitidis, Streptococcus pneumoniae, Enterobacteriaceae, Staphylococcus aureus, Candida spp., and seven other pathogens. The MB stain yielded the highest detection rate, 99%, in comparison with 94 and 93% for the AO and G stains, respectively. Since the MB stain yielded comparable results to the AO procedure with no requirement for a fluorescent microscope, the former method is recommended for confirming the presence and initial characterization of microorganisms in blood cultures monitored by Bactec from children. <\/jats:p>","DOI":"10.1139\/m83-119","type":"journal-article","created":{"date-parts":[[2010,5,20]],"date-time":"2010-05-20T18:30:15Z","timestamp":1274380215000},"page":"729-731","source":"Crossref","is-referenced-by-count":1,"title":["The detection of positive blood cultures in children using the acridine orange, gram, and methylene blue stains"],"prefix":"10.1139","volume":"29","author":[{"given":"Leonard J. La","family":"Scolea Jr.","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan M.","family":"Baldigo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m83-119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T05:12:15Z","timestamp":1578287535000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m83-119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1983,6,1]]}},"alternative-id":["10.1139\/m83-119"],"URL":"https:\/\/doi.org\/10.1139\/m83-119","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"type":"print","value":"0008-4166"},{"type":"electronic","value":"1480-3275"}],"subject":[],"published":{"date-parts":[[1983,6,1]]}}}