{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T01:00:29Z","timestamp":1649120429626},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"2","license":[{"start":{"date-parts":[[1989,2,1]],"date-time":"1989-02-01T00:00:00Z","timestamp":602294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1989,2,1]]},"abstract":"<jats:p> The IncHII plasmid pHH1508a (208 kilobases) encodes resistance to potassium tellurite, trimethoprim, and streptomycin. Conjugative pili encoded by pHH1508a were isolated, purified, and used for preparation of anti-H pilus antiserum. Immuno-gold labelling experiments using H pilus specific antiserum showed that antigenic determinants were located along the entire length of the H pilus. Immuno-gold labelling and lysis studies using pilH\u03b1, a bacteriophage specific for H pili, were used to investigate transfer-deficient mutants of pHH1508a obtained by Tn5 mutagenesis and an in vitro constructed derivative of 96 kilobases, pDT1178, which also conferred resistance to potassium tellurite, trimethoprim, and streptomycin. The transfer-deficient mutants did not specify H pili, whereas pDT1178, which transferred at low frequency (1\u2002\u00d7\u200210<jats:sup>\u22124<\/jats:sup> transconjugants per recipient), specified a small number of H pili. A naturally occurring plasmid, pMG110, was found to encode the production of H pili, but was completely transfer deficient (&lt;\u20021\u2002\u00d7\u200210<jats:sup>\u22127<\/jats:sup> transconjugants per recipient). This study suggests that genes required for H pilus production and assembly as well as low level transfer are located separately within the 96-kilobase fragment of pDT1178 and that other genes, located outside this region, are essential for the regulation and full expression of conjugative transfer.Key words: plasmid, incompatibility, mapping, antibiotic, resistance. <\/jats:p>","DOI":"10.1139\/m89-043","type":"journal-article","created":{"date-parts":[[2009,11,13]],"date-time":"2009-11-13T16:37:54Z","timestamp":1258130274000},"page":"289-294","source":"Crossref","is-referenced-by-count":1,"title":["Mapping of transfer and H pilus coding regions of the IncHII plasmid pHH1508a"],"prefix":"10.1139","volume":"35","author":[{"given":"William","family":"Yan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane E.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m89-043","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T05:13:46Z","timestamp":1578287626000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m89-043"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1989,2,1]]}},"alternative-id":["10.1139\/m89-043"],"URL":"https:\/\/doi.org\/10.1139\/m89-043","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,2,1]]}}}