{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:47:37Z","timestamp":1757706457713},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[1990,4,1]],"date-time":"1990-04-01T00:00:00Z","timestamp":638928000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1990,4,1]]},"abstract":"<jats:p> The isotypic immune response of 16 individuals who developed Q fever pneumonia following exposure to an infected parturient cat was studied. The enzyme-linked immunosorbent (ELISA) test was used to detect IgM, IgA, and IgG antibodies to phase I and phase II Coxiella burnetii whole-cell antigens and to the phase I lipopolysaccharide. The indirect immunofluorescent antibody (IFA) test was also used to detect antibodies to phase I and phase II whole cells. None of the 16 subjects developed antibodies to the phase I lipopoly saccharide. The ELISA was more sensitive than the IFA test. IgM antibodies to phase II antigen were detectable by ELISA in 80% of the subjects at the time of onset of symptoms and were still present in 7 of the 8 tested at 32 weeks following the onset of symptoms. In all instances (ELISA: IgG, IgM; IFA: IgG, IgM) phase II antibodies developed earlier and reached higher levels than did phase I antibodies. The absence of antibodies to phase I lipopolysaccharide in acute Q fever combined with our unpublished findings of antibodies to phase I lipopoly saccharide in chronic Q fever suggests that this test may be used to distinguish acute from chronic Q fever. Key words: Q fever, immune response, ELISA. <\/jats:p>","DOI":"10.1139\/m90-050","type":"journal-article","created":{"date-parts":[[2010,5,20]],"date-time":"2010-05-20T14:39:32Z","timestamp":1274366372000},"page":"292-296","source":"Crossref","is-referenced-by-count":18,"title":["The immune response in a cat-related outbreak of Q fever as measured by the indirect immunofluorescence test and the enzyme-linked immunosorbent assay"],"prefix":"10.1139","volume":"36","author":[{"given":"J.","family":"Embil","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. C.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. J.","family":"Marrie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m90-050","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T06:52:41Z","timestamp":1578207161000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m90-050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1990,4,1]]}},"alternative-id":["10.1139\/m90-050"],"URL":"https:\/\/doi.org\/10.1139\/m90-050","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,4,1]]}}}