{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T22:21:45Z","timestamp":1726438905761},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"1","license":[{"start":{"date-parts":[[1991,1,1]],"date-time":"1991-01-01T00:00:00Z","timestamp":662688000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1991,1,1]]},"abstract":"<jats:p> The association of a 20-MDa plasmid with nalidixic acid resistant (Nal<jats:sup>r<\/jats:sup>) strains of Shigella dysenteriae 1 has been examined. The plasmid, which is readily transferable, does not itself code for nalidixic acid resistance but offers a survival advantage to its host under nalidixic acid stress. The plasmid-containing cultures of S. dysenteriae 1 produced Nal<jats:sup>r<\/jats:sup> mutants in vitro at a frequency 1000-fold higher than their plasmidless parent strains, after two exposures to nalidixic acid. Using a similar procedure, mutants resistant to other antibiotics such as chloramphenicol, tetracycline, or ciprofloxacin could not be isolated. The genome of S. dysenteriae 1 appears to carry a heavy load of the insertion sequence IS1. The propensity of the plasmid-containing strains to readily mutate to nalidixic acid resistance and its possible relevance to the observed association of the plasmid with Nal<jats:sup>r<\/jats:sup> clinical isolates is discussed. Key words: Shigella, nalidixic acid, plasmid, shigellosis. <\/jats:p>","DOI":"10.1139\/m91-009","type":"journal-article","created":{"date-parts":[[2010,5,20]],"date-time":"2010-05-20T14:41:07Z","timestamp":1274366467000},"page":"59-63","source":"Crossref","is-referenced-by-count":16,"title":["Unusual association of a plasmid with nalidixic acid resistance in an epidemic strain of <i>Shigella dysenteriae<\/i> type 1 from Asia"],"prefix":"10.1139","volume":"37","author":[{"given":"M. Musharraf","family":"Ashraf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zia U.","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Sack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m91-009","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T06:52:34Z","timestamp":1578207154000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m91-009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,1,1]]}},"alternative-id":["10.1139\/m91-009"],"URL":"https:\/\/doi.org\/10.1139\/m91-009","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"type":"print","value":"0008-4166"},{"type":"electronic","value":"1480-3275"}],"subject":[],"published":{"date-parts":[[1991,1,1]]}}}