{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:50:45Z","timestamp":1791251445486,"version":"4.1.0"},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"7","license":[{"start":{"date-parts":[[1991,7,1]],"date-time":"1991-07-01T00:00:00Z","timestamp":678326400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1991,7,1]]},"abstract":"<jats:p> Eleven hundred Bacillus strains isolated from marine sediment from the Minas Basin, Nova Scotia, Canada, were purified on LB agar supplemented with ampicillin, chloramphenicol, erythromycin, streptomycin, tetracycline, or mercuric chloride. Seventy-seven isolates were examined for plasmid DNA, and for resistance to 11 antibiotics, HgCl<jats:sub>2<\/jats:sub>, and phenylmercuric acetate. Minimum inhibitory concentrations of Ag, Cd, Co, Cu, and Zn were also determined. Forty-three percent of antibiotic- and mercury-resistant strains contained one or more plasmids ranging from 1.9 to 210\u2002MDa. Fifty-four percent carried plasmids greater than 20\u2002MDa, and 97% were resistant to two or more metals. There was no correlation between plasmid content and resistance either to antibiotics or to mercurial compounds in these strains. Mercury-resistant isolates were unable to transform Hg<jats:sup>2+<\/jats:sup> to volatile Hg<jats:sup>0<\/jats:sup> by virtue of a mercuric reductase enzyme system (mer). Strains resistant to Hg<jats:sup>2+<\/jats:sup> were investigated for their ability to produce H<jats:sub>2<\/jats:sub>S and intracellular acid-labile sulfide when grown in the absence and presence of HgCl<jats:sub>2<\/jats:sub>. Lower levels of H<jats:sub>2<\/jats:sub>S and intracellular sulfide were detected only in metal-resistant strains grown in the presence of HgCl<jats:sub>2<\/jats:sub>, suggesting that cellular sulfides complexed with Hg<jats:sup>2+<\/jats:sup> in these strains. Key words: Bacillus, plasmid, antibiotic resistance, metal resistance, marine sediment. <\/jats:p>","DOI":"10.1139\/m91-087","type":"journal-article","created":{"date-parts":[[2010,5,20]],"date-time":"2010-05-20T14:41:44Z","timestamp":1274366504000},"page":"513-520","source":"Crossref","is-referenced-by-count":42,"title":["Occurrence of antibiotic and metal resistance and plasmids in <i>Bacillus<\/i> strains isolated from marine sediment"],"prefix":"10.1139","volume":"37","author":[{"given":"B. H.","family":"Belliveau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. E.","family":"Starodub","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. T.","family":"Trevors","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m91-087","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T06:51:43Z","timestamp":1578207103000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m91-087"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,7,1]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1991,7,1]]}},"alternative-id":["10.1139\/m91-087"],"URL":"https:\/\/doi.org\/10.1139\/m91-087","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,7,1]]}}}