{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T09:03:46Z","timestamp":1695891826581},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"12","license":[{"start":{"date-parts":[[1991,12,1]],"date-time":"1991-12-01T00:00:00Z","timestamp":691545600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1991,12,1]]},"abstract":"<jats:p> Cryptosporidum sp. from guinea pigs and C. parvum were compared morphologically, electrophoretically, and for the ability to infect suckling mice. Oocysts from guinea pigs measured 5.4\u2002\u00d7\u20024.6 (4.8\u20135.6\u2002\u00d7\u20024.0\u20135.0)\u2002\u03bcm and had a shape index (length\/width) of 1.17 (1.04\u20131.33). Oocysts of C. parvum were similar and measured 5.2\u2002\u00d7\u20024.6 (4.8\u20135.6\u2002\u00d7\u20024.2\u20134.8)\u2002\u03bcm with a shape index of 1.16 (1.04\u20131.33). All suckling mice inoculated with oocyts of C. parvum became infected, whereas most, but not all, mice fed oocysts of the guinea pig isolate also became infected. However, mice inoculated with oocysts from guinea pigs produced on average 100-fold fewer oocysts by day 7 postinoculation than did mice infected with C. parvum, and the resulting infections were sparse and patchy along the ileum. Electrophoretic profiles were similar, but <jats:sup>125<\/jats:sup>I surface labeling of outer oocyst wall proteins revealed striking differences between the two isolates. Cryptosporidium parvum had a wide molecular size range of <jats:sup>125<\/jats:sup>I-labeled bands, whereas C. sp. from guinea pigs had a banding pattern clustered between 39 and 66\u2002kDa, with a smaller number of bands\u2002&gt;100\u2002kDa. Key words: Cryptosporidium parvum, coccidia, Apicomplexa, guinea pig, mouse. <\/jats:p>","DOI":"10.1139\/m91-163","type":"journal-article","created":{"date-parts":[[2010,5,20]],"date-time":"2010-05-20T18:42:52Z","timestamp":1274380972000},"page":"949-952","source":"Crossref","is-referenced-by-count":19,"title":["A comparative study on the biology of <i>Cryptosporidium<\/i> sp. from guinea pigs and <i>Cryptosporidium parvum<\/i> (Apicomplexa)"],"prefix":"10.1139","volume":"37","author":[{"given":"Michael","family":"Tilley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve J.","family":"Upton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clarence E.","family":"Chrisp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"fr","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m91-163","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T11:51:19Z","timestamp":1578225079000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m91-163"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,12,1]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1991,12,1]]}},"alternative-id":["10.1139\/m91-163"],"URL":"https:\/\/doi.org\/10.1139\/m91-163","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,12,1]]}}}