{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T02:03:02Z","timestamp":1760061782530},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"2","license":[{"start":{"date-parts":[[1993,2,1]],"date-time":"1993-02-01T00:00:00Z","timestamp":728524800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1993,2,1]]},"abstract":"<jats:p> A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly purified preparations of the enzyme showed a single band on SDS polyacrylamide gel electrophoresis with a molecular weight of 70\u2002000. The isolated enzyme had a molecular weight of about 200\u2002000 as determined by gel filtration and disc gel electrophoresis. These results suggest an apparently trimeric structure of the enzyme. Dithiothreitol treatment disrupted the trimer into a dimeric structure of 140\u2002000. Along with ageing, as well as limited proteolytic digestion, the enzyme evolved to give a dimeric molecule of 105\u2002000 composed of two identical subunits of 52\u2002000. The combination of the purified enzyme with HgCl<jats:sub>2<\/jats:sub>, or unexpectedly with merthiolate, oxidised the NADPH, which was followed spectrophotometrically. The K<jats:sub>m<\/jats:sub> for HgCl<jats:sub>2<\/jats:sub> was dependent on the concentration of exogenous thiol compounds. A comparison of physical properties as well as kinetic characteristics indicated that the enzyme from Y. enterocolitica 138A14 is similar to mercuric reductases isolated from other mercury-resistant bacteria.Key words: Yersinia enterocolitica, mercury resistance, mercuric reductase. <\/jats:p>","DOI":"10.1139\/m93-027","type":"journal-article","created":{"date-parts":[[2010,5,20]],"date-time":"2010-05-20T18:45:43Z","timestamp":1274381143000},"page":"193-200","source":"Crossref","is-referenced-by-count":10,"title":["Purification and properties of mercuric reductase from <i>Yersinia enterocolitica<\/i> 138A14"],"prefix":"10.1139","volume":"39","author":[{"given":"Mohamed","family":"Blaghen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique J. M.","family":"Vidon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Said El","family":"Kebbaj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m93-027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T11:51:25Z","timestamp":1578225085000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m93-027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,2,1]]}},"alternative-id":["10.1139\/m93-027"],"URL":"https:\/\/doi.org\/10.1139\/m93-027","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,2,1]]}}}