{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T21:47:04Z","timestamp":1648936024104},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"12","license":[{"start":{"date-parts":[[1993,12,1]],"date-time":"1993-12-01T00:00:00Z","timestamp":754704000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[1993,12,1]]},"abstract":"<jats:p> The infectivity of Borrelia burgdorferi depends on the number of passages in Barbour\u2013Stoenner\u2013Kelly (BSK-II) medium in ambient air. In this study the parameters of medium formulation or O<jats:sub>2<\/jats:sub>\u2013CO<jats:sub>2<\/jats:sub> atmosphere were altered to define conditions that would retain borrelial infectivity upon serial passage. The infective strain Sh-2-82 was passaged 20 times in BSK-II and BSK-A (a modified BSK-II medium) in ambient O<jats:sub>2<\/jats:sub>\u2013CO<jats:sub>2<\/jats:sub> and BSK-II in 4% O<jats:sub>2<\/jats:sub> \u2013 5% CO<jats:sub>2<\/jats:sub> \u2013 91% N<jats:sub>2<\/jats:sub>. Spirochetes of every fifth passage were inoculated intraperitoneally into neonatal Lewis rats. Infectivity was lost after 15 passages in media in ambient O<jats:sub>2<\/jats:sub>\u2013CO<jats:sub>2<\/jats:sub>. However, infectivity was maintained through all 20 passages in BSK-II in 4% O<jats:sub>2<\/jats:sub> \u2013 5% CO<jats:sub>2<\/jats:sub> \u2013 91% N<jats:sub>2<\/jats:sub>. There were no pH differences, but the dissolved O<jats:sub>2<\/jats:sub> concentration in ambient O<jats:sub>2<\/jats:sub>\u2013CO<jats:sub>2<\/jats:sub> was approximately twice that in 4% O<jats:sub>2<\/jats:sub> \u2013 5% CO<jats:sub>2<\/jats:sub> \u2013 91% N<jats:sub>2<\/jats:sub>. Therefore, differences in infectivity were due to culturing under a constant environment of decreased O<jats:sub>2<\/jats:sub> and increased CO<jats:sub>2<\/jats:sub>. This environment may place a selective pressure on the borreliae for retention of the infective phenotype. The results suggest that the levels of O<jats:sub>2<\/jats:sub> and CO<jats:sub>2<\/jats:sub> in the environment influence infectivity by preventing the loss of genetic information or inducing the expression of virulence determinants in B. burgdorferi.Key words: Borrelia burgdorferi; Lyme disease, infectivity, serial passage. <\/jats:p>","DOI":"10.1139\/m93-167","type":"journal-article","created":{"date-parts":[[2010,5,20]],"date-time":"2010-05-20T18:47:00Z","timestamp":1274381220000},"page":"1103-1110","source":"Crossref","is-referenced-by-count":8,"title":["Maintenance of infective <i>Borrelia burgdorferi<\/i> Sh-2-82 in 4% oxygen \u2013 5% carbon dioxide in vitro"],"prefix":"10.1139","volume":"39","author":[{"given":"Faye E.","family":"Austin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"fr","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/m93-167","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T11:50:05Z","timestamp":1578225005000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/m93-167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12,1]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1993,12,1]]}},"alternative-id":["10.1139\/m93-167"],"URL":"https:\/\/doi.org\/10.1139\/m93-167","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,12,1]]}}}