{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T04:15:23Z","timestamp":1751516123723,"version":"3.41.0"},"reference-count":26,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p> Researchers hope to increase \u03b3-globin expression by controlling potential trans-acting factors that specifically activate the \u03b3-globin gene in fetuses or silence this gene in adults to potentially treat sickle cell disease and \u03b2-thalassemias. To characterize genes encoding such factors, we analyzed the differential expression of mRNAs in erythroid induction cultures of CD34<jats:sup>+<\/jats:sup> cells derived from normal adult bone marrow, umbilical cord blood, and bone marrow from a patient with heterocellular hereditary persistence of fetal hemoglobin. Using differential-display \u2013 reverse-transcription PCR analysis, we identified a number of genes with differential expression in the above-mentioned cells. The differential expression of some genes was also confirmed by quantitative real-time PCR. Our data provide important clues for identifying and validating trans-activators that activate the \u03b3-globin gene in fetuses, and trans-acting factors involved in silencing the \u03b3-globin gene in adults. <\/jats:p>","DOI":"10.1139\/o07-007","type":"journal-article","created":{"date-parts":[[2007,6,18]],"date-time":"2007-06-18T20:31:20Z","timestamp":1182198680000},"page":"347-357","source":"Crossref","is-referenced-by-count":1,"title":["Screening for <i>trans<\/i>-acting factors and other factors involved in the activating or silencing of the \u03b3-globin gene during human ontogeny"],"prefix":"10.1139","volume":"85","author":[{"given":"Yan-Ni","family":"Ma","sequence":"first","affiliation":[{"name":"National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People\u2019s Republic of China."},{"name":"The 303rd hospital, Nanning, People\u2019s Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People\u2019s Republic of China."},{"name":"The 303rd hospital, Nanning, People\u2019s Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-Wu","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People\u2019s Republic of China."},{"name":"The 303rd hospital, Nanning, People\u2019s Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-Hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People\u2019s Republic of China."},{"name":"The 303rd hospital, Nanning, People\u2019s Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong-Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People\u2019s Republic of China."},{"name":"The 303rd hospital, Nanning, People\u2019s Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"atypb1\/ref1","doi-asserted-by":"crossref","first-page":"3571","DOI":"10.1128\/MCB.19.5.3571","volume":"19","author":"Asano H.","year":"1999","journal-title":"Mol. 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