{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T09:11:34Z","timestamp":1778663494528,"version":"3.51.4"},"reference-count":81,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p> Bothrops snake venoms cause renal damage, with renal failure being the main cause of death in humans bitten by these snakes. In this work, we investigated the cytoskeletal rearrangement and cytotoxicity caused by Bothrops alternatus venom in cultured Madin\u2013Darby canine kidney (MDCK) cells. Incubation with venom (10 and 100\u00a0\u00b5g\/mL) significantly (p\u00a0&lt;0.05) decreased the cellular uptake of neutral red dye after 1\u00a0and 3\u00a0h. Venom (100\u00a0\u00b5g\/mL) also markedly decreased the transepithelial electrical resistance (R<jats:sub>T<\/jats:sub>) across MDCK monolayers. Staining with rhodamine-conjugated phalloidin revealed disarray of the cytoskeleton that involved the stress fibers at the basal cell surface and focal adhesion-associated F-actin in the cell\u2013matrix contact region. Feulgen staining showed a significant decrease in the number of cells undergoing mitosis and an increase in the frequency of altered nuclei. Scanning electron microscopy revealed a decrease in the number of microvilli and the presence of cells with a fusiform format. Flow cytometry with annexin V and propidium iodide showed that cell death occurred by necrosis, with little apoptosis, a conclusion supported by the lack of DNA fragmentation characteristic of apoptosis. Pretreating the cells with catalase significantly attenuated the venom-induced loss of viability, indicating a possible involvement of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> in the cellular damage; less protection was observed with superoxide dismutase or N<jats:sup>\u03c9<\/jats:sup>-nitro-l-arginine methyl ester. These results indicate that Bothrops alternatus venom is cytotoxic to cultured MDCK cells, possibly via the action of reactive oxygen species. This cytotoxicity could contribute to nephrotoxicity after envenoming by this species. <\/jats:p>","DOI":"10.1139\/o07-067","type":"journal-article","created":{"date-parts":[[2007,9,27]],"date-time":"2007-09-27T18:02:19Z","timestamp":1190916139000},"page":"591-605","source":"Crossref","is-referenced-by-count":23,"title":["Cytoskeletal rearrangement and cell death induced by <i>Bothrops alternatus<\/i> snake venom in cultured Madin\u2013Darby canine kidney cells"],"prefix":"10.1139","volume":"85","author":[{"given":"Juliana\u00a0Minardi","family":"Nascimento","sequence":"first","affiliation":[{"name":"Departamento de Farmacologia, Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas (UNICAMP), CP 6111, 13083-970, Campinas, SP, Brazil."},{"name":"Departamento de Bioqu\u00edmica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brazil."},{"name":"Centro Integrado de Pesquisas Oncohematol\u00f3gicas Infantis (CIPOI), Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas (UNICAMP), CP 6111, 13083-970, Campinas, SP, Brazil."},{"name":"Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilberto\u00a0Carlos","family":"Franchi","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas (UNICAMP), CP 6111, 13083-970, Campinas, SP, Brazil."},{"name":"Departamento de Bioqu\u00edmica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brazil."},{"name":"Centro Integrado de Pesquisas Oncohematol\u00f3gicas Infantis (CIPOI), Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas (UNICAMP), CP 6111, 13083-970, Campinas, SP, Brazil."},{"name":"Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre\u00a0Eduardo","family":"Nowill","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas (UNICAMP), CP 6111, 13083-970, Campinas, SP, Brazil."},{"name":"Departamento de Bioqu\u00edmica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brazil."},{"name":"Centro Integrado de Pesquisas Oncohematol\u00f3gicas Infantis (CIPOI), Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas (UNICAMP), CP 6111, 13083-970, Campinas, SP, Brazil."},{"name":"Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla\u00a0Beatriz","family":"Collares-Buzato","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas (UNICAMP), CP 6111, 13083-970, Campinas, SP, Brazil."},{"name":"Departamento de Bioqu\u00edmica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brazil."},{"name":"Centro Integrado de Pesquisas Oncohematol\u00f3gicas Infantis (CIPOI), Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas (UNICAMP), CP 6111, 13083-970, Campinas, SP, Brazil."},{"name":"Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Hyslop","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas (UNICAMP), CP 6111, 13083-970, Campinas, SP, Brazil."},{"name":"Departamento de Bioqu\u00edmica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brazil."},{"name":"Centro Integrado de Pesquisas Oncohematol\u00f3gicas Infantis (CIPOI), Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Estadual de Campinas (UNICAMP), CP 6111, 13083-970, Campinas, SP, Brazil."},{"name":"Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"atypb1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(85)90379-4"},{"key":"atypb2\/ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-006-7959-9"},{"key":"atypb3\/ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00249-5"},{"key":"atypb4\/ref4","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(02)00156-3"},{"key":"atypb5\/ref5","doi-asserted-by":"crossref","first-page":"751","DOI":"10.4269\/ajtmh.1991.45.751","volume":"45","author":"Barral-Netto M.","year":"1991","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"atypb6\/ref6","first-page":"243","volume":"45","author":"Bauab F.A.","year":"1994","journal-title":"Trop. Med. Parasitol."},{"key":"atypb7\/ref7","doi-asserted-by":"crossref","first-page":"698","DOI":"10.4269\/ajtmh.1999.61.698","volume":"61","author":"Boer-Lima P.A.","year":"1999","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"atypb8\/ref8","doi-asserted-by":"crossref","first-page":"217","DOI":"10.4269\/ajtmh.2002.67.217","volume":"67","author":"Boer-Lima P.A.","year":"2002","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"atypb9\/ref9","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3390481"},{"key":"atypb10\/ref10","doi-asserted-by":"crossref","first-page":"82","DOI":"10.4269\/ajtmh.1993.48.82","volume":"48","author":"Burdmann E.A.","year":"1993","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"atypb11\/ref11","first-page":"315","volume":"86","author":"Cardoso J.L.","year":"1993","journal-title":"Q. J. Med."},{"key":"atypb12\/ref12","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00067"},{"key":"atypb13\/ref13","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(98)00111-1"},{"key":"atypb14\/ref14","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1016\/j.toxicon.2004.03.015","volume":"43","author":"Castro I.","year":"2004","journal-title":"Toxicon"},{"key":"atypb15\/ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-006-0078-9"},{"key":"atypb16\/ref16","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4583"},{"key":"atypb17\/ref17","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/0167-4889(94)90163-5","volume":"1222","author":"Collares-Buzato C.B.","year":"1994","journal-title":"Biochim. Biophys. Acta"},{"key":"atypb18\/ref18","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0171-9335(98)80020-4","volume":"76","author":"Collares-Buzato C.B.","year":"1998","journal-title":"Eur. J. Cell Biol."},{"key":"atypb19\/ref19","doi-asserted-by":"publisher","DOI":"10.1006\/taap.2002.9404"},{"key":"atypb20\/ref20","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9861(03)00298-4"},{"key":"atypb21\/ref21","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M311771200"},{"key":"atypb22\/ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.08.009"},{"key":"atypb23\/ref23","first-page":"185","volume":"16","author":"Cruz-H\u00f6fling M.A.","year":"2001","journal-title":"Histol. Histopathol."},{"key":"atypb24\/ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.11.014"},{"key":"atypb25\/ref25","doi-asserted-by":"publisher","DOI":"10.1080\/713803463"},{"key":"atypb26\/ref26","first-page":"2613","volume":"27","author":"Domingos M.O.","year":"1994","journal-title":"Braz. J. Med. Biol. Res."},{"key":"atypb27\/ref27","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(02)00102-2"},{"key":"atypb28\/ref28","unstructured":"Fran\u00e7a, F.O.S., and M\u00e1laque, C.M.S. 2003. Acidente botr\u00f3pico. In: Animais pe\u00e7onhentos no Brasil: biologia, cl\u00ednica e terap\u00eautica dos acidentes. Edited by J.L.C. Cardoso, F.O.S. Fran\u00e7a, F.H. Wen, C.M.S. M\u00e1laque, and V. Haddad Jr. Sarvier\/FAPESP, S\u00e3o Paulo, Brazil. pp.\u00a072\u201386."},{"key":"atypb29\/ref29","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(03)90158-1"},{"key":"atypb30\/ref30","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.124.4.619"},{"key":"atypb31\/ref31","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(91)90106-2"},{"key":"atypb32\/ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.06.019"},{"key":"atypb33\/ref33","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(95)00085-Z"},{"key":"atypb34\/ref34","unstructured":"Guti\u00e9rrez, J.M., and Lomonte, B. 2003. Efectos locales en el envenenamiento of\u00eddico en Am\u00e9rica Latina. In Animais pe\u00e7onhentos no Brasil: biologia, cl\u00ednica e terap\u00eautica dos acidentes. Edited by J.L.C. Cardoso, F.O.S. Fran\u00e7a, F.H. Wen, C.M.S. M\u00e1laque, and V. Haddad Jr. Sarvier\/FAPESP, S\u00e3o Paulo. pp.\u00a0310\u2013323."},{"key":"atypb35\/ref35","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2003.11.005"},{"key":"atypb36\/ref36","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)01163-9"},{"key":"atypb37\/ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.02.029"},{"key":"atypb38\/ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2003.11.011"},{"key":"atypb39\/ref39","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00146-5"},{"key":"atypb40\/ref40","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(99)01667-0"},{"key":"atypb41\/ref41","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(91)90079-7"},{"key":"atypb42\/ref42","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1590\/S0100-879X1998000700001","volume":"31","author":"Kamiguti A.S.","year":"1998","journal-title":"Braz. J. Med. Biol. Res."},{"key":"atypb43\/ref43","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S0026-895X(24)12251-1","volume":"59","author":"Ling Y.","year":"2001","journal-title":"Mol. Pharmacol."},{"key":"atypb44\/ref44","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4538"},{"key":"atypb45\/ref45","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(94)90408-1"},{"key":"atypb46\/ref46","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2003.11.008"},{"key":"atypb47\/ref47","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03867.x"},{"key":"atypb48\/ref48","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6728"},{"key":"atypb49\/ref49","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2530036.x"},{"key":"atypb50\/ref50","doi-asserted-by":"publisher","DOI":"10.1016\/0020-711X(70)90077-7"},{"key":"atypb51\/ref51","unstructured":"Mebs, D. 1978. Pharmacology of reptilian venoms. In Biology of the reptilia. Physiology. Vol. 8B. Edited by C. Gans and K.A. Gans. Vol.\u00a08B. Academic Press, London. pp.\u00a0437\u2013560."},{"key":"atypb52\/ref52","unstructured":"Melgarejo, A.R. 2003. Serpentes pe\u00e7onhentas do Brasil. In Animais pe\u00e7onhentos no Brasil: biologia, cl\u00ednica e terap\u00eautica dos acidentes. Edited by J.L.C. Cardoso, F.O.S. Fran\u00e7a, F.H. Wen, C.M.S. M\u00e1laque, and V. Haddad Jr. Sarvier\/FAPESP, S\u00e3o Paulo. pp.\u00a033\u201361."},{"key":"atypb53\/ref53","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1091\/mbc.4.9.953","volume":"4","author":"Meredith J.","year":"1993","journal-title":"Mol. Biol. Cell"},{"key":"atypb54\/ref54","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.02.012"},{"key":"atypb55\/ref55","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(88)90009-8"},{"key":"atypb56\/ref56","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(90)90094-N"},{"key":"atypb57\/ref57","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(91)90063-W"},{"key":"atypb58\/ref58","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(86)90151-0"},{"key":"atypb59\/ref59","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00229-X"},{"key":"atypb60\/ref60","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(03)00005-1"},{"key":"atypb61\/ref61","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.bbagen.2006.11.015","volume":"1770","author":"Ponce-Soto L.A.","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"atypb62\/ref63","first-page":"407","volume":"22","author":"Rezende N.A.","year":"1989","journal-title":"Braz. J. Med. Biol. Res."},{"key":"atypb63\/ref64","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(92)90505-Y"},{"key":"atypb64\/ref65","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1590\/S0100-879X2005001000007","volume":"38","author":"Selistre-de-Araujo H.S.","year":"2005","journal-title":"Braz. J. Med. Biol. Res."},{"key":"atypb65\/ref66","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(98)80008-1"},{"key":"atypb66\/ref67","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2000.2120"},{"key":"atypb67\/ref68","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2004.03.049"},{"key":"atypb68\/ref69","first-page":"298","volume":"73","author":"Staiano N.","year":"1997","journal-title":"Eur. J. Cell Biol."},{"key":"atypb69\/ref70","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/0305-0491(91)90387-S","volume":"100","author":"Tan N.H.","year":"1991","journal-title":"Comp. Biochem. Physiol. B"},{"key":"atypb70\/ref72","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(94)90206-2"},{"key":"atypb71\/ref73","doi-asserted-by":"crossref","first-page":"593","DOI":"10.4269\/ajtmh.1992.47.593","volume":"47","author":"Theakston R.D.G.","year":"1992","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"atypb72\/ref74","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1981.tb15490.x"},{"key":"atypb73\/ref75","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022414503995"},{"key":"atypb74\/ref76","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1590\/S0100-879X1998000300017","volume":"31","author":"Vasconcelos C.M.L.","year":"1998","journal-title":"Braz. J. Med. Biol. Res."},{"key":"atypb75\/ref77","unstructured":"Warrell, D.A. 2004 Snakebites in Central and South America: epidemiology, clinical features and clinical management. In Venomous reptiles of the western hemisphere. Vol. 2. Edited by J. A. Campbell and W. W. Lamar. Ithaca. Comstock Publishing Associates\/Cornell University Pres, Ithaca, N.Y. pp.\u00a0709\u2013761."},{"key":"atypb76\/ref78","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1995.65"},{"key":"atypb77\/ref79","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(03)00183-6"},{"key":"atypb78\/ref80","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3570719"},{"key":"atypb79\/ref81","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(03)00105-8"},{"key":"atypb80\/ref83","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00123-4"},{"key":"atypb81\/ref85","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S1388-1981(02)00122-1","volume":"1581","author":"Zhao S.","year":"2002","journal-title":"Biochim. Biophys. Acta"}],"container-title":["Biochemistry and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/O07-067","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/O07-067","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T18:03:24Z","timestamp":1751479404000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/O07-067"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":81,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1139\/O07-067"],"URL":"https:\/\/doi.org\/10.1139\/o07-067","relation":{},"ISSN":["0829-8211","1208-6002"],"issn-type":[{"value":"0829-8211","type":"print"},{"value":"1208-6002","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10]]}}}