{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:21:12Z","timestamp":1791354072885,"version":"4.3.1"},"reference-count":56,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[2008,8,1]],"date-time":"2008-08-01T00:00:00Z","timestamp":1217548800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:p>A cDNA encoding the vitellogenin receptor of the ixodid tick, Haemaphysalis longicornis Neumann (HlVgR) was cloned and characterized. The full-length cDNA is 5631\u00a0bp, including an intact ORF encoding an expected protein with 1782\u00a0amino acids. The deduced amino acid sequence of the HlVgR cDNA revealed two ligand-binding domains with four class A cysteine-rich repeats in the first domain and eight in the second domain similar to those of insect VgRs. The immunoblot analysis detected ~197\u00a0kDa protein in both tick ovary and egg. The developmental expression profile demonstrated that HlVgR mRNA exists throughout the ovarian development, and the transcriptional level is especially high in the previtellogenic period. Immuno electron microscopy analysis demonstrated that the localization of HlVgR is detected on the external surface of oocyte plasma membrane. RNAi showed that eggs of HlVgR dsRNA-injected adult ticks had not developed into fully mature oocytes and laid abnormal eggs. The Babesia parasite DNA was not detected in the eggs of HlVgR dsRNA-injected tick that fed on Babesia gibsoni infected dog, whereas it was detected in the eggs of PBS-injected ticks and noninjected ticks. Expression of HlVgR was increased by the vitellogenic hormone 20-hydroxyecdysone. These results indicate that HlVgR, which is produced by the developing oocytes, is essential for Vg uptake, egg development in the H. longicornis tick, and transovarial transmission of Babesia parasites.<\/jats:p>","DOI":"10.1139\/o08-071","type":"journal-article","created":{"date-parts":[[2008,8,28]],"date-time":"2008-08-28T14:55:25Z","timestamp":1219935325000},"page":"331-344","source":"Crossref","is-referenced-by-count":68,"title":["Tick vitellogenin receptor reveals critical role in oocyte development and transovarial transmission of\n                    <i>Babesia<\/i>\n                    parasite"],"prefix":"10.1139","volume":"86","author":[{"given":"Damdinsuren","family":"Boldbaatar","sequence":"first","affiliation":[{"name":"Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima\u00a0890-0065, Japan."},{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro\u00a0080-8555, Hokkaido, Japan."},{"name":"Division of Tropical Disease and Parasitology, Kyorin University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badgar","family":"Battsetseg","sequence":"additional","affiliation":[{"name":"Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima\u00a0890-0065, Japan."},{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro\u00a0080-8555, Hokkaido, Japan."},{"name":"Division of Tropical Disease and Parasitology, Kyorin University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohide","family":"Matsuo","sequence":"additional","affiliation":[{"name":"Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima\u00a0890-0065, Japan."},{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro\u00a0080-8555, Hokkaido, Japan."},{"name":"Division of Tropical Disease and Parasitology, Kyorin University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Hatta","sequence":"additional","affiliation":[{"name":"Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima\u00a0890-0065, Japan."},{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro\u00a0080-8555, Hokkaido, Japan."},{"name":"Division of Tropical Disease and Parasitology, Kyorin University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rika","family":"Umemiya-Shirafuji","sequence":"additional","affiliation":[{"name":"Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima\u00a0890-0065, Japan."},{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro\u00a0080-8555, Hokkaido, Japan."},{"name":"Division of Tropical Disease and Parasitology, Kyorin University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuenan","family":"Xuan","sequence":"additional","affiliation":[{"name":"Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima\u00a0890-0065, Japan."},{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro\u00a0080-8555, Hokkaido, Japan."},{"name":"Division of Tropical Disease and Parasitology, Kyorin University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kozo","family":"Fujisaki","sequence":"additional","affiliation":[{"name":"Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima\u00a0890-0065, Japan."},{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro\u00a0080-8555, Hokkaido, Japan."},{"name":"Division of Tropical Disease and Parasitology, Kyorin University School of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"rg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(85)90080-3"},{"key":"rg2\/ref2","unstructured":"Agbede, R.I.S., Kemp, D.H., and Hoyte, H.M.D. 1986\n                      a\n                      . 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Arnold Press, London. pp.\u00a0349\u2013359."},{"key":"rg52\/ref52","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2005.05.011"},{"key":"rg53\/ref53","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2005.00570.x"},{"key":"rg54\/ref54","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2006.11.007"},{"key":"rg55\/ref55","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150180314"},{"key":"rg56\/ref56","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.01.037"}],"container-title":["Biochemistry and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/O08-071","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/O08-071","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T13:52:21Z","timestamp":1751464341000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/O08-071"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,8]]},"references-count":56,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,8]]}},"alternative-id":["10.1139\/O08-071"],"URL":"https:\/\/doi.org\/10.1139\/o08-071","relation":{},"ISSN":["0829-8211","1208-6002"],"issn-type":[{"value":"0829-8211","type":"print"},{"value":"1208-6002","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,8]]}}}