{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T19:39:15Z","timestamp":1769283555583,"version":"3.49.0"},"reference-count":41,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:p> Different cell types have been reported to internalize lactoferrin (Lf) by specific or nonspecific receptors. Our studies focused on the endocytic pathway of human Lf in macrophage-like THP-1 cells. Lactoferrin was found to be internalized by THP-1 cells differentiated with phorbol myristate acetate. Incubation of cells with chlorpromazine and dansylcadaverine, inhibitors of clathrin-dependent endocytosis, led to a 50% inhibition of Lf internalization compared with untreated cells. Bafilomycin A1 and NH<jats:sub>4<\/jats:sub>Cl treatment also resulted in 40%\u201360% inhibition, respectively, suggesting that the internalization of Lf may partly be mediated by acidic endosome-like organelles. Endocytic uptake of Lf was also cholesterol-dependent, as shown by methyl-\u03b2-cyclodextrin or nystatin treatment of the cells prior to internalization. Partial colocalization of Lf and EEA-1, a marker specific for early endosomes, could be observed. Colocalization of Lf with a specific endoplasmic reticulum marker was also detected. Our results suggest that Lf is internalized mainly by the clathrin-dependent pathway in THP-1 cells and targets the ER. The physiological consequences of this intracellular trafficking will be the subject of future investigations. <\/jats:p>","DOI":"10.1139\/o11-090","type":"journal-article","created":{"date-parts":[[2012,5,16]],"date-time":"2012-05-16T17:27:29Z","timestamp":1337189249000},"page":"449-455","source":"Crossref","is-referenced-by-count":15,"title":["Endocytosis and trafficking of human lactoferrin in macrophage-like human THP-1 cells<sup>1<\/sup>This article is part of a Special Issue entitled Lactoferrin and has undergone the Journal\u2019s usual peer review process."],"prefix":"10.1139","volume":"90","author":[{"given":"Paula","family":"Florian","sequence":"first","affiliation":[{"name":"Institute of Biochemistry of the Romanian Academy, Spl. Independentei 296, Bucharest 17 060031, Romania."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alina","family":"Macovei","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry of the Romanian Academy, Spl. 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