{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T09:32:41Z","timestamp":1762075961719},"reference-count":11,"publisher":"Canadian Science Publishing","issue":"7","license":[{"start":{"date-parts":[[1967,7,1]],"date-time":"1967-07-01T00:00:00Z","timestamp":-79056000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem."],"published-print":{"date-parts":[[1967,7,1]]},"abstract":"<jats:p>Insulin exhibited some cross-reactivity with the isolated S-sulfo A- and B-chains in the passive cutaneous anaphylaxis test. The synthetic peptides A<jats:sub>1\u20139<\/jats:sub>, A<jats:sub>10\u201321<\/jats:sub>, and A<jats:sub>1\u201321<\/jats:sub>gave a passive cutaneous anaphylactic reaction with guinea pig antiserum to natural A-chain, whereas high levels of A<jats:sub>10\u201321<\/jats:sub>and A<jats:sub>1\u201321<\/jats:sub>could also inhibit a subsequent insulin challenge from reacting with antiserum to insulin. The synthetic peptides B<jats:sub>1\u20138<\/jats:sub>, B<jats:sub>9\u201330<\/jats:sub>, B<jats:sub>24\u201330<\/jats:sub>, and B<jats:sub>1\u201330<\/jats:sub>reacted with antiserum to natural B-chain but high levels of peptides (B<jats:sub>2\u20138<\/jats:sub>)<jats:sub>2<\/jats:sub>, B<jats:sub>9\u201314<\/jats:sub>, and (B<jats:sub>17\u201323<\/jats:sub>)<jats:sub>2<\/jats:sub>were inactive. The symmetrical double peptide (B<jats:sub>1\u20138<\/jats:sub>)<jats:sub>2<\/jats:sub>reacted with guinea pig antiserum to intact insulin as well as with antiserum to B-chain and certain sera from insulin-resistant diabetic patients. The results indicated that injection of ox insulin into guinea pigs caused production of anaphylactic antibodies mainly towards antigenic loci in the regions A<jats:sub>10\u201321<\/jats:sub>and B<jats:sub>1\u20138<\/jats:sub>of the insulin molecule, whereas antigenic determinants in the regions A<jats:sub>1\u20139<\/jats:sub>and B<jats:sub>24\u201330<\/jats:sub>were less important.<\/jats:p>","DOI":"10.1139\/o67-130","type":"journal-article","created":{"date-parts":[[2009,12,17]],"date-time":"2009-12-17T22:08:40Z","timestamp":1261087720000},"page":"1135-1144","source":"Crossref","is-referenced-by-count":14,"title":["THE ANTIGENIC LOCI OF INSULIN"],"prefix":"10.1139","volume":"45","author":[{"given":"S.","family":"Wilson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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